OR08-6 Encapsulated Thermogenic and Appetite Suppressing Fibroblasts Attenuate Weight Gain in Mice

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: OR08-Obesity: Novel Mechanisms of Body Weight Regulation
Saturday, June 15, 2013: 11:30 AM-1:00 PM
Presentation Start Time: 12:45 PM
Room 301 (Moscone Center)
David Joel DiSilvestro*1, Ouliana Ziouzenkova2 and Kichoon Lee1
1The Ohio State University, Columbus, OH, 2Ohio State Univ, Columbus, OH
Obesity affects about 30% of Americans causing health care costs to rise.  Novel treatment methods are needed to combat the obesity problem when tradition methods, such as diet and exercise, fail.  Therapies involving the implantation of thermogenic cells into adipose tissue induce the host’s immune response and may cause the host to compensate weight loss with increased food consumption.  To overcome this problem, we encapsulated thermogenic cells in a porous alginate-poly-l-lysine (APL) membrane that enables long-term cell survival in the host’s tissue.  We hypothesized that injecting these capsules, which contain thermogenic and appetite suppressing cells, into adipose tissue would increase energy metabolism and stimulate weight loss. To test this, we generated two sets of thermogenic capsules. We knocked out raptor in 3T3-L1 cells (RKO).  To control appetite, we stably transfected an aliquot of RKO with leptin and amylin to generate a new cell line (LAR).  We determined that RKO and LAR were prime candidates for thermogenic capsules because these lines had greater glucose uptake than 3T3-L1 cells in vitro.  To test in vivo, 12 week old wild-type female mice were fed a high-fat (HF) diet (45% kcal from fat) for 132 days and then injected with cellular or acellular APL capsules.  The control group (Empty­­SV) received acellular capsule injections into both subcutaneous (S) and visceral (V) fat pads.  The second group (RVEmptyS) received RKO capsule injections into the visceral fat pads and acellular capsule injections in the subcutaneous fat pads. The final group (RVLARS) received RKO ­­capsules in the visceral fat and LAR capsules in the subcutaneous fat pads.  After 51 days on a HF diet, the percent weight gain of the RVLARS was significantly less than the percent weight gain of the EmptySV; likewise, the percent weight gain of the RVEmptyS was less than the percent weight gain of the Empty­­SV.  These results show the efficacy of these capsules for increasing thermogenesis and reducing appetite in the attenuation of weight gain induced by a HF diet.

Nothing to Disclose: DJD, OZ, KL

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm

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