Omega-6 Fatty Acid Modulates MiRNA Profile in Microgravity Cultured Adipocytes

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: SUN 649-677-Adipocyte Biology
Sunday, June 16, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board SUN-664
Nalini Santanam*, Holly Tamski and Carla Cook
Marshall University School of Medicine, Huntington, WV
Adipose tissue plays an important role in metabolic processes. Alteration in adipose function increases risk to cardiometabolic diseases. Several physiological and environmental factors including diet, aging, and environment modulate adipose function. These factors trigger the activation of epigenetic regulators such as microRNAs (small non-coding RNAs) which modulate gene expression. A better understanding of these physiological and environmental factors that regulate adipose function will help in improving our understanding of adipose biology. We recently identified that aging associated adipose dysfunction is related to changes in key miRNAs in the adipose derived stem cells. In the present study we investigated the effect of dietary omega-6 fatty acids on miRNA profile in adipocytes cultured in microgravity. Both 3T3-L1 and human pre-adipocytes or differentiated adipocytes were either cultured in normal or zero gravity in the presence or absence of 25-50 µM of linoleic acid (key dietary omega-6 fatty acid). Mouse and human whole genome miRNome miScript miRNA PCR array (v16.0) was performed using Roche LightCycler 480 system. Preliminary analysis of the miRNA profile revealed >50% of miRNAs were down-regulated in cells cultured in microgravity. Dramatic increases in miRNAs were observed in adipocytes cultured in the presence of omega-6 fatty acid, with highest changes in cells grown in microgravity. Our data provides evidence for diet and environment to alter epigenetic changes in adipose tissue. Pathway analysis will be used to classify miRNAs relevant to adipose function which will help identify miRNAs to explore in future research.

Nothing to Disclose: NS, HT, CC

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Sources of Research Support: NASA WV EPScoR