The Effects of Turmeric (Curcumin) on Tumor Suppresser Protein (p53) and Estrogen Receptor (ERá) in Breast Cancer Cells

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: MON 355-388-Sex Hormone Receptor Action & Reaction
Basic/Translational
Monday, June 17, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board MON-377
Nada Sitto*1, Amy Elizabeth Siebert1, Maria S Yonan1, Lisa Shammas1, Michelle Nguyen2, Bryan Allender1, Omar Atcho1, Meghan Quigley1 and Sumi Dinda1
1Oakland University, Rochester, MI, 2Oakland University, MI
Curcumin (diferuloylmethane) is a polyphenol, the essential component found in the spice turmeric.  This powerful culinary spice has substantial biological effects in different systems and has recently captivated attention for having antibacterial, antiviral, anti-inflammatory, and anticancer properties. A significant number of studies have been published on the effects of curcumin on the regulation of genes, which are known to contribute to tumorigenesis, cell proliferation, invasion, and angiogenesis.  In this study, we have analyzed the effects of curcumin on the expression of p53 and ERα with the presence of estrogen, BPA and anti-estrogens in T47D breast cancer cells. Cells were cultured in medium containing 5% charcoal-stripped fetal bovine serum for 6 days in order to deplete any endogenous steroids or effectors. The cells were then treated for 24 hours with 60 μM curcumin, which was determined to be the optimal value by a concentration study of curcumin ranging from 5-100 µM. Protein was extracted from the cells, quantified, and subjected to SDS-PAGE and Western blot analysis.  For functional analysis, alterations in T47D cell proliferation were quantified upon exposure to curcumin. The treatment with curcumin alone caused a 10-fold decrease in cell proliferation compared to the estrogen-treated cells, which suggests its anti-proliferative effects. Western blot analysis revealed a relative decrease in the levels of p53 and ERα upon treatment with 5-60 µM when compared to untreated controls. These effects are sensitive to the presence of antiestrogens.  However, cytolocalization of p53 and ERα upon treatment with estradiol and curcumin remained unaltered.  Delineating the role of curcumin in the regulation of p53 with ERα and their mechanisms of action may be important in understanding the influence of curcumin on tumor suppressors and hormone receptors in breast cancer.

Nothing to Disclose: NS, AES, MSY, LS, MN, BA, OA, MQ, SD

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