The Effect of GH replacement therapy (GHRT) on soluble Klotho in patients with GH deficiency (GHD)

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: SAT 109-133-GHRH, GH & IGF Biology & Signaling
Bench to Bedside
Saturday, June 15, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board SAT-118
Rebecca Locher*1, Andrea Egger2, Cornelia Zwimpfer3, Lisa Sze4, Christoph Schmid5 and Emanuel R Christ6
1University Hospital of Zurich, Zürich, Switzerland, 2University Hospital of Berne, Berne, Switzerland, 3University Hospital of Zurich, Zurich, Switzerland, 4Cantonal Hospital of St. Gallen, St. Gallen, Switzerland, 5Univ Zurich, Zurich, Switzerland, 6University Hosp of Berne, Berne, Switzerland
Background

Soluble Klotho (sKl) levels are markedly elevated in patients with acromegaly and decline with successful surgical therapy suggesting a causal relationship between GH-excess and sKl. It is unknown, whether (i) GH-deficient patients (GHD) have lower sKl levels than control subjects and (ii) GH replacement therapy (GHRT) results in an increase in sKl concentrations. We, therefore, measured sKI levels in GH-deficient patients before and after GHRT and in sedentary healthy controls (CS) matched for gender, age, BMI and waist.

Methods:

Ten patients with GHD (4 female and 6 male patients; age:42±12.5, mean±SD, years; BMI: 26.6±3.8, kg/m2; waist:89.3±12.9, cm) and ten healthy controls (age: 42.8±12.6; BMI: 25.2±5.3; waist:90.7±19.1) were included in this study. Identical investigations (anthrompometric and biochemical evaluation) were performed in the GHD (before and after 6 months GHRT) and in the CS. Serum sKl was measured by an ELISA, IGF-1 concentrations by Immunolite (SIEMENS).

Results:

Anthropometric data were not significatly different between GHD and CS. GHRT did not result in a significant change in BMI or waist. GHD exhibited a reduced IGF- concentration compared to CS (GHD: median (IQR): 68.5 ng/mL (46.9–68.2) vs CS: 111.5 (98.8 – 130.3); p<0.005). sKI concentrations were not significantly different between GHD and CS. GHRT resulted in a significant increase in IGF-1 levels (GHD: 68.5 ng/mL (46.9-68.2) vs GHRT: 153.0 (117.3-149.5; p<0.002) and sKI concentrations (GHD:497 pg/mL (419-658) vs GHRT 692 (590-1233); p=0.002)

Conclusion

These findings (i) confirm a causal relationship between GH and sKl but (ii) show that sKl is not particularly low in GHD (not significantly different from CS).

Nothing to Disclose: RL, AE, CZ, LS, CS, ERC

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm