Multiplex Analysis of High Molecular Weight Adiponectin and 18 Other Biomarkers of Obesity in Human Serum and Plasma

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: SUN 690-701-Obesity Pathophysiology
Sunday, June 16, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board SUN-692
James David, Michael Anderson* and Jane Schmidt
R&D Systems, Inc., Minneapolis, MN
The rapidly increasing prevalence of obesity in developed countries worldwide has increased research interest in its causes, prevention and treatment.  As with many chronic diseases, chronic inflammation plays a role in the development of the metabolic problems and pathological conditions associated with obesity. Adipose tissue itself is now recognized as an endocrine organ releasing proteins that are referred to as adipocytokines.  Adiponectin is an important adipocytokine that circulates in distinct low, mid, and high molecular weight forms with differing metabolic effects. The concentration of high molecular weight (HMW) Adiponectin in plasma is negatively correlated with conditions related to obesity such as insulin resistance, dyslipidemia, coronary artery disease, and atherogenesis.  HMW Adiponectin is also the most potent isoform at inducing insulin sensitization in the liver.  We have developed a multiplex immunoassay for the Luminex® platform for simultaneous measurement of either total or HMW Adiponectin with 17 other biomarkers of obesity in serum or plasma.  In this study we compare the levels of HMW Adiponectin, total Adiponectin, Dipeptidyl Peptidase IV (DPPIV or CD26), Chitinase 3-like 1 (CHI3L1 or YKL-40), C-Reactive Protein (CRP), FGF-21, Follistatin-Related Gene Protein (FLRG), Hepatocyte Growth Factor (HGF), Plasminogen Activator Inhibitor-1 (PAI-1 or Serpin E1), Adipsin (Complement Factor D), Resistin, Leptin, Interleukin-1β (IL-1β), Interleukin-6 (IL-6), Interleukin-10 (IL-10), Receptor for Advanced Glycation Endproducts (RAGE), Sex Hormone Binding Globulin (SHBG), Tumor Necrosis Factor-α (TNF-α), and Monocyte Chemotactic Protein 1 (MCP-1) in serum or plasma from individual samples across a range of BMI levels.  Multiplex analysis of these biomarkers provides an efficient tool to assist in studies to define the biology of conditions associated with obesity.

Disclosure: JD: Employee, R&D Systems, Inc.. MA: Employee, R&D Systems, Inc.. JS: Employee, R&D Systems, Inc..

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