Session: SAT 1-25-Glucocorticoid Actions & HPA Axis
Poster Board SAT-8
Results: We have examined the impact of GCs or hypothermia (HT) on cell fate specification, using NSCs exposed to Dex or HT only during their proliferative phase and then withdrawn from hormone or changed to normothermia upon the initiation of differentiation. The number and type of cells produced from each lineage were examined following 5-14 days of differentiation using indirect immunofluorescence to detect markers of neurons (neuron specific class III beta-tubulin) and glia, (glial fibrillary acidic protein). Preliminary results showed that GC treatment during proliferation alters the fate of NSCs leading to enhancement of neurogenesis at a time when untreated cells were mainly adopting a glial identity. Furthermore, Dex alters neurogenic fate as evidenced by the increased production of bipolar neurons and decreased multi-polar neurons generated upon differentiation of early passage progenitors. Hypothermia alone did not lead to these changes in neurogenic fate.
Conclusion: Future studies will include more detailed analysis of GC effects on genes responsible for triggering gliogenesis and examination of hormone effects on NSCs with distinct cell fates.
Nothing to Disclose: LFG, DT, SFW, PM, DBD
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