RECENTLY DIAGNOSED PEDIATRIC PATIENTS WITH TYPE 1 DIABETES HAVE A DECREASED PERCENTAGE OF NKT CELLS

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: MON 596-630-Pediatric Endocrinology
Clinical
Monday, June 17, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board MON-606
Rita A. Gomez-Diaz*1, Mayra Vaal Aguilar2, Nonantzin Beristain-Cobarrubias3, Abril Arellano Llama2, Vianney Ortiz Navarrete3, Rocio Herrera Márquez2, Elisa Nishimura Meguro2, Eulalia Garrido Magaña2, Adan Valladares Salgado2, Raul Silva2, Rafael Mondragon Gonzalez2, Carolina Bekker Méndez2 and Niels Wacher2
1IMSS,, Mexico DF, Mexico, 2IMSS, 3CINVESTAV
RECENTLY DIAGNOSED PEDIATRIC PATIENTS WITH TYPE 1 DIABETES HAVE A DECREASED PERCENTAGE OF NKT CELLS

Gómez-Díaz RA1, Vaal Aguilar M1, Beristain-Cobarrubias N2, Arellano Llama A3, Ortiz Navarrete V2, Herrera Márquez R3, Nishimura Meguro E3, Garrido Magaña E3, Valladares Salgado A4, Silva R3, Mondragon Gonzalez R1, Bekker Méndez C5, Wacher N1.

1Unidad de Investigación en Epidemiología Clínica and 4Bioquímica, UMAE Hospital de Especialidades, 3Hospital de Pediatría del CMN Siglo XXI. Instituto Mexicano del Seguro Social y el 2Departamento de Biomedicina Molecular del CINVESTAV del Instituto Politécnico Nacional. 5Unidad de Investigación en Inmunología e Infectología del Hospital de Infecto, CMN "La Raza" IMSS. México. D.F.

INTRODUCTION.

In type 1 diabetes, a failure in the regulation of either innate or acquired immunity may be the cause of autoimmune response. One cell population that may have a regulatory role of the immune response are the Natural Killer T (NKT) cells, which are a population expressing T lymphocyte antigen receptor (TCR), and a common marker for NK cells. A decrease in the number and/or functional incapability of NKT cells is associated with progression of type 1 diabetes and with other self-immune diseases.

OBJECTIVE.

To compare the percentage of NKT cells in pediatric patients with type 1 diabetes and their first-degree relatives (parents and siblings) against healthy subjects matched for age and gender.

MATERIAL AND METHODS.

This is an analytical cross-sectional study of pediatric patients recently diagnosed with type 1 diabetes (maximum 3 months evolution), first-degree relatives (parents and siblings) and control families.  The study included analysis of NKT cells, predictor antibodies and HLA risk and protector haplotypes.

RESULTS.

The mean percentage of NKT cells was lower in the recently diagnosed cases (0.03% (95%CI 0.01-0.74) compared against controls (0.12% (0.02-0.75), p=0.015). No difference was observed when the same comparison was done against the relatives of the two groups. More than half of the patients (54.5%) with T1D showed positive levels of antibodies (anti-GAD, anti-IA2, anti-insulin). Of these, 40.9% were positive for one antibody and 13.6% for at least two of the three. 81.8% of the patients showed risk allele DR3 and/or DR4 and 95.5% of the patients were positive for risk alleles DQ2, DQ3.

CONCLUSIONS.

There are differences in the percentage of NKT between patients with recently-diagnosed T1D compared with apparently healthy controls.

KEYWORDS: NKT cells, HLA, pediatric diabetes, Type 1 diabetes, antibodies

Nothing to Disclose: RAG, MV, NB, AA, VO, RH, EN, EG, AV, RS, RM, CB, NW

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm