The immunoexpression of CYP17 in the ovary of the melatonin-treated pinealectomized rats

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: MON 561-585-Ovarian & Uterine Function II
Basic/Clinical
Monday, June 17, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board MON-569
Sylvia Yamashita Hayashida*1, Carla Maganhin2, Edmund Chada C. Baracat1, Gisela Sato3, Maria Candida Baracat1, Ricardo Simoes1 and Jose Maria Soares Jr1
1Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo, Brazil, 2UNIFESP, São Paulo, São Paulo, Brazil, 3UNIFESP, Sao Paulo, Brazil
Objective: to analyze the immunoexpresssion of CYP17, which is an enzime related to the pregnenolone and androstenedione synthesis in the ovary of pinealectomized (Px) rat after the  melatonin (Me) treatment. Design: Twenty adult virgin female rats (Rattus norvegicus albinus) derived from the Bioterium of UNIFESP and with regular estrous cycle, were Px and divided into two groups: GPx (treated with vehicle solution) and GPxMe - orally treated with Me (10 µg/night/animal), during 60 consecutive days. After treatments, the rats were euthanized and the ovaries were collected and processed for histological routine. Part of histological sections (5µm) were stained with hematoxilin and eosin (H.E) and other were submitted to immunohistochemical reaction for the CYP17 detection. Under light microscopy and with the Software Image Pro Plus, the density of the immunoreaction was quantified. Data were subjected to the Mann Whitney U test for statistical analyses (p<0.05). Results: In the H.E stained sections, The ovarian mature follicles and corpora lutea were not found in the ovaries of the GPx, while we identified ovarian follicles in various developmental stages, including the preovulatory follicles and the presence of corpora lutea in the GPxMe,. In addition, the CYP17 immunoexpresion of the GPxMe ovary was lower than one of GPx (GPx =50.3 ± 0.8 and GPxMe = 40.9 ± 0.9*, p<0.05). Conclusion: The administration of melatonin decreases the immunoexpression of CYP17 in the ovary of pinealectomized rats.

Nothing to Disclose: SYH, CM, ECCB, GS, MCB, RS, JMS

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