Session: MON 548-560-Hyperandrogenic Disorders
Poster Board MON-555
Methods: For cell sizing studies, ~0.5g frozen SC abdominal adipose was embedded in OCT, cut into 10μm sections, stained with H&E following standard protocols, and digitally imaged. mRNA gene expression, determined by qRT-PCR and expressed as Delta Ct values, included adipogenic genes Zfp423; C/EBPα and PPARδ; and PPARλ as markers of adipocyte stem cell commitment to preadipocytes, early-stage preadipocyte differentiation, and late-stage preadipocyte progression, respectively.
Results: Adipocyte area (AA), adipocyte circumference (AC) and the AA/AC ratio were similar between female groups. In T-exposed vs. C females, Zfp423 mRNA expression was increased (p<0.025), while C/EBPα mRNA expression was reciprocally diminished (p<0.003); PPARδ and PPARλ mRNA expression was comparable between female groups. In a subset of C (n=3) and T-exposed (n=5) females with similar volume of SC abdominal adipose by female group, a positive correlation was found between Zfp423 mRNA expression and area-under-the-curve total free fatty acids during an intravenous glucose tolerance test (p<0.008, r2=0.66, all females combined). Adipogenic gene expression with these selected markers was not associated with adipocyte morphology, glucoregulatory parameters, or animal BMI or age.
Conclusion: Early gestation T exposure may perturb SC abdominal adipogenesis during transition of adipocyte stem cell commitment to early preadipocyte differentiation, thereby constraining SC abdominal lipid storage as a mechanism of promoting lipotoxicity in PCOS women as they age.
Nothing to Disclose: GDC, PA, EK, DAD, DHA
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