Delay in the onset of puberty of intrauterine growth retarded female rats cannot be rescued with hypernutrition after birth

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: MON 596-630-Pediatric Endocrinology
Monday, June 17, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board MON-625
Toshiya Matsuzaki*, Ganbat Gereltsetseg, Takeshi Iwasa, Riyo Kinouchi and Minoru Irahara
Department of Obstetrics and Gynecology, The University of Tokushima Graduate School, Institute of Health Biosciences, Tokushima, Japan
Perinatal undernutrition is known to disturb reproductive development, in particular by delaying the onset of

puberty in certain species. Using a rat model, we studied whether hypernutrition after birth can rescue the delayed onset

of puberty in intrauterine undernourished female rats. Pregnant rats were divided into two groups: the maternal normal

nutrition (mNN, n = 8) and maternal undernutrition (mUN, n = 9) groups. In the mUN group, dams received 50% of the

daily food intake of the mNN group from day 15 of pregnancy until delivery. Pups from both the mNN and mUN dams

were then separated into two groups, based on their postnatal feeding conditions: control-normal nutrition (control-NN),

control-hypernutrition (control-HN), Intrauterine growth retardation (IUGR)-normal nutrition (IUGR-NN), and IUGRhypernutrition

(IUGR-HN). Litter sizes of the hypernutrition groups were controlled to five pups per dam, and normal

nutrition groups to 12-13 pups per dam. From postnatal day 30, pups were inspected daily for vaginal opening (VO). The

age of VO in the IUGR-NN group was 35.7 ± 2.4 days (mean ± SD), which was significantly delayed compared to that of

the control-NN group (33.8 ± 0.8 days). The age of VO in the IUGR-HN group was 35.5 ± 2.3 days, which was significantly

delayed compared to that of the control-HN group (33.5 ± 0.8 days). Interestingly, the age of VO did not differ between

the IUGR-NN and IUGR-HN groups. In conclusion, maternal undernutrition delays puberty in female offspring, and this

delay in puberty cannot be rescued with hypernutrition after birth.

(1) Iwasa T, Matsuzaki T, Murakami M, Fujisawa S, Kinouchi R, Gereltsetseg G, Kuwahara A, Yasui T, Irahara M (2010) Effects of intrauterine undernutrition on hypothalamic Kiss1 expression and the timing of puberty in female rats. J Physiol 588(5): 821-829.(2) Iwasa T, Matsuzaki T, Murakami M, Shimizu F, Kuwahara A, Yasui T, Irahara M (2008) Decreased expression of kisspeptin mediates acute immune/ inflammatory stress-induced suppression of gonadotropin secretion in female rat. J Endocrinol Invest 31: 656- 659.(3) Tortoriello DV, McMinn J, Chua SC (2004) Dietaryinduced obesity and hypothalamic infertility in female DBA/2J mice. Endocrinology 145: 1238-1247.

Nothing to Disclose: TM, GG, TI, RK, MI

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