Session: FP15-Adipocyte Biology
Bench to Bedside
Room 303 (Moscone Center)
Poster Board SUN-666
To investigate interactions between fat cells and HIV-infected memory CD4 T cells, purified human memory CD4+ T cells were infected with HIV in vitro and cocultured in transwells with primary subcutaneous preadipocytes or mature adipocytes for up to 1 week. CD4 T cell functions known to regulate HIV replication such as activation, proliferation, and survival were examined by flow cytometry. Both preadipocytes and adipocytes enhanced T cell activation and HIV production within 3-6 days of coculture. The enhanced CD4 T cell activation and increased HIV production were mediated by IL6 and integrin ligands from preadipocytes and adipocytes. Intriguingly, there was an absolute requirement for common γ-chain cytokines IL2, IL7, or IL15 (exogenously added to cocultures), which are important for homeostatic stimulation and survival of memory T cells. IL7 and IL15 mRNA expression (but not IL2 mRNA) was detected in preadipocytes and adipocytes. Interestingly, mature adipocytes expressed both IL15 and IL15-receptor-α protein on their cell surfaces, but did not secrete IL15 into the medium, suggesting that adipocytes may “trans-present” IL15 to HIV-infected CD4 T cells via cell contact, and thus engender the signals that enhance HIV replication. Both uninfected and HIV-infected memory CD4 T cells also inhibited adipogenesis. These results demonstrate key interactions between HIV-infected memory CD4 T cells and fat cells that increase T cell activation and HIV replication with a block in adipogenesis.
Nothing to Disclose: JC, DJL, AB, DEL
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