Session: MON 112-141-Hypothalamus-Pituitary Development & Biology
Poster Board MON-122
In this study, we started to generate specific antibodies that are able to recognize each PRRX1 and PRRX2, and succeeded in production of the antibodies specific for each. We first analyzed temporospatial localization of PRRX1 and PRRX2 by immunohistochemistry using novel specific antibodies as well as SOX2 and some vascular markers, such as isolectin B4. The results indicated that PRRX1+ cells were present in the anterior and intermediate lobes, especially on the marginal cell layer postulated as a stem cell niche from late embryonic stage to early postnatal stage. PRRX1 co-localized with SOX2 in the marginal cell layer of the anterior and intermediate lobes, and was observed in a few hormone-positive cells in the parenchyma of the anterior lobe. In contrast, PRRX2+ cells were not detected at embryonic stage but were definitely observed within some of SOX2+ cells lining the marginal cell layer at postnatal stage. In addition, each PRRX1+ and PRRX2+cell was present in the surrounding mesenchyme and invaded the embryonic pituitary, followed by differentiation into vascular endothelial and smooth muscle cells.
In conclusion, analyses of the temporospatial localization pattern of PRRX1 and PRRX2 in rat pituitary development successfully demonstrated that PRRX1 and PRRX2 are present in the undifferentiated and transient differentiated hormone-producing cells and also in the surrounding mesenchymal cells, followed by differentiation into hormone-producing cells and vascular cells.
Nothing to Disclose: MH, SY, MT, SS, HU, MS, HY, NK, MC, TK, YK
*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm
See more of: Abstracts - Orals, Featured Poster Presentations, and Posters