Association of rs7901695 and rs12255372 of TCF7L2 gene with Gestational Diabetes Mellitus and its relationship with metabolic and hormonal characteristics in Mexican women

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: SAT 806-823-Gestational Diabetes
Saturday, June 15, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board SAT-810
Ruth Lopez-Reyes*1, Juan M Malacara2 and Elva L Perez-Luque3
1Universidad de Guanajuato, Leon, Mexico, 2Univ de Guanajuato, Leon Gto, Mexico, 3Univ de Guanajuato, Leon Guanajuato, Mexico
Introduction: Gestational Diabetes Mellitus (GDM) was defined as glucose intolerance with onset or first recognition during pregnancy whether or not the condition persisted after pregnancy and predispose to Type 2 Diabetes Mellitus (T2DM). Variation in transcription factor 7-like 2 (TCF7L2) gene has been shown to be associated with type 2 diabetes and with GDM in several populations.

Objective: To determine the association of genetic variants rs7903146 y rs12255372 of TCF7L2 gene with Gestational Diabetes Mellitus and its relationship with metabolic and hormonal parameters.

Material and Methods: We selected 108 pregnant women with normal glucose tolerance (NGT) and 90 diagnosed with GDM according to ADA criterion matched for age and gestational week. We collected blood pressure, body mass index (BMI), blood glucose, HbA1c lipids, insulin and GLP-1. The genotyping of rs7903146 and rs12255372 polymorphisms were made by means of PCR-RFLP. Data are presented as means ± ED or median (25th - 75th), Chi-square analysis was used to test for difference in genotype and allele frequencies between GDM and control groups. Logistic regression analysis was used to calculate the age-adjusted and/or crude ORs and 95% CIs for the polymorphisms.

Results: Actual and pre-gestational weight and BMI, as well as fasting glucose, and HBA1c were higher (p<0.001), and HDL-Cholesterol was lower (p=0.02) in DMG women than NTG women. No significant differences were found for lipids, insulin, and HOMAIR). However, the GDM women had high GLP-1 levels (32 [21 – 43] vs 24 [16-34] p=0.004) and decreased b-cell function (266 [170-408] vs 438 [317-767], p<0.001). The frequency of rs12255372 in GDM women was significantly higher than in NGT women (X2=10.29; p=0.001). Logistic regression analysis showed that HbA1c, and GLP-1 levels are associated factors (OR=3.4 [95% CI 2.2–5.1], p<0.001) and (OR=3.6 [95% CI 1.01–12.91], p=0.04). The presence of rs12255372T allele confers a risk for GDM (OR=7.2, [95% CI 2.96–17.53], p<0.001). The presence of rs7903146T allele confers a risk for GDM (OR=1.9 [95% CI 1.03–3.51], p=0.03).

Conclusions: We found elevated lipid levels normally observed in pregnancy. Insulin levels were similar in both groups, but GLP-1 levels were significantly elevated in GDM women. This suggests that women with GDM need more GLP-1 to reach the insulin levels observed in the NTG women. Additional, the β-cell function was lower in GDM women as observed in type 2 DM. The rs12255372T allele confers risk for GDM. Age, BMI and HOMA-IR are also risk factors independently associated with the development GDM.

Nothing to Disclose: RL, JMM, ELP

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