Session: MON 723-757-Renin-Angiotensin-Aldosterone System/Endocrine Hypertension
Bench to Bedside
Poster Board MON-738
Methods : Five blood samples from each patients were collected into EDTA tubes at the room temperature. The first sample was processed within 30 minutes (control group). Other two blood samples were processed after freezing and thawing in ice bath (0°C) or room temperature (Group 2 and Group 3). The last two samples were kept in ice bath (0°C) or room temperature (25°C) for 6 hours then freezing and thawing in ice bath before PRA assay (Group 4 and Group 5).
Results :Two-hundred samples from 40 patients were collected for matched analysis. In control group, PRA was 0.635 ng/ml/hr. Freezing and thawing in ice bath or room temperature can significantly increase PRA, 1.805 (p<0.001) and 1.470 (p<0.001) ng/ml/h, respectively. The blood samples which were kept in ice bath (0°C) or room temperature (25°C) for 6 hours significantly increased PRA , 2.055 ng/ml/hr (p<0.001) and 1.915 ng/ml/hr (p=0.001) . There were no significant differences when group 2 and group 3 (p=0.181) or group 4 and group 5 (p=0.610) were compared. The ARR calculated from the sample with cryoactivation was significantly decreased from the baseline value which resulted in falsely negative results.
Conclusion : Thawing process of plasma caused significant increment of plasma renin activity, both in ice bath(0°C) or at room temperature (25°C). There were no differences of PRA results between specimen collected in ice bath or room temperature for 6 hours. Therefore, the interpretation of PRA results from samples exposed to cold temperature, even in short period during thawing process, should be done with caution.
Nothing to Disclose: SS, NP
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