Protein Biomarkers in Identification of Cardiovascular Risk Factors in Hypertensive Prepubertal Children

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: MON 723-757-Renin-Angiotensin-Aldosterone System/Endocrine Hypertension
Bench to Bedside
Monday, June 17, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board MON-740
Nabila Roohi*1 and Ateek Ahmad2
1Department of Zoology (Endocrinology), University of the Punjab, Lahore, Pakistan, 2Department of Pediatrics, Lahore General Hospital, Lahore, Pakistan
Background and aims: Hypertension during childhood is the most prevalent risk factor for cardiovascular disease (CVD) and resultant morbidities and mortalities in adult life. Early identification of these children is of great significance in the prevention of future cardiovascular events.

Subjects and Methods: Participants were hypertensive (SBP and DBP>95th percentile) prepubertal boys (n=22; Mean age:10.21±0.36 years; BMI 5th to <85th percentile) and their normotensive (SBP and DBP<90th percentile) counterparts (n=22; Mean age:10.62±0.41 years; BMI 5th to <85th percentile) with negative family history of hypertension and/or CVD. We assessed plasma proteome by two dimensional gel electrophoresis using Protean IEF coupled with II XL Cell System. The resolved proteins were  identified by Swiss 2DPAGE database.

Results: Comparative proteome analysis indicated fourteen differentially expressed protein spots in hypertensive children than their normotensive counterparts. Nine of these spots were up-regulated, whereas, five were down-regulated in hypertensive compared to normal children. The expressions of Apolipoprotein (Apo) A-I, ApoA-IV, ApoE, Albumin and Transthyretin were significantly suppressed (p<0.05) in 55%, 27%, 41%, 23% and 59% of hypertensive children, respectively. ApoB-100, C-reactive protein, Ceruloplasmin, Complement factor C3, Retinol binding protein, Fibronectin, Fibrinogen beta chain, Prothrombin and Plasminogen activator inhibitor-1 indicated marked expressions (p<0.05) in 68%, 64%, 45%, 14%, 45%, 27%, 55%, 20% and 50% of hypertensive children, respectively. Most of these proteins are apolipoproteins, clotting factors or inflammatory markers. Altered expressions of these proteins predict that children with hypertension, irrespective of their family history of hypertension and/or CVD, are at a higher risk of future progression towards cardiovascular disorders.

Conclusions: Variedly expressed plasma proteins, in children with hypertension, may serve as novel diagnostic biomarkers for tracking the progression of morbidities in adulthood. Moreover, extensive blood pressure control strategies, at population level, are mandatory to reduce the consequences of hypertension and associated health risks in these children. Effective lifestyle modifications, improved diet and physical activity may be helpful in the prevention and reversal of hypertension. Long term follow up studies are required to observe the effectiveness of prevention strategies.  

Nothing to Disclose: NR, AA

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