Session: MON 818-841-Diabetes Pathophysiology & Complications
Poster Board MON-832
Byung Soo Kong1, Yoon Hee Cho1, and Eun Jig Lee 1
1Endocrinology, Severance Integrative Research Institute for Cerebral & Cardiovascular Diseases, Yonsei University College of Medicine, Seoul, Korea
Protocatechuic aldehyde (PCA) is a single compound found in aqueous extract of Salvia Miltiorrhiza that have recently been reported for its antioxidative effects in atherosclerosis. Numerous studies suggest that two estrogen receptors, ER α and ER β, have beneficial cardiovascular effects. However, effects of G-protein coupled estrogen receptor (GPER-1), the third membrane bound estrogen receptor, on atherosclerosis are not well understood. Here, we report that PCA reduces vascular inflammation through increasing GPER-1 expression at mRNA and protein level in the similar manner as GPER-1 agonist, G1.
In order to find out the anti-atherogenic effects of PCA, HUVECs were pretreated with PCA and G1 and then, tumor necrosis factor alpha (TNF-α) was given. PCA and G1 prevented the TNF-α stimulated inflammatory process by reducing VCAM-1, ICAM-1, and HIF1-α expression in HUVECs. Subsequently, reduced phosphorylation of p-38 MAPK and Nf-kB were observed by treatment of PCA and G1.
In addition, HUVECs were treated with G15, an antagonist of GPER-1, and its GPER-1 gene was silenced to elucidate the effects of GPER-1 and PCA on endothelial cell inflammation. Increased expression of VCAM-1 and ICAM-1 were observed from both groups. However, PCA and G1 decreased the expression of these adhesion molecules. Our results demonstrated that PCA has anti-atherogenic effects by activating GPER-1 as G1 and by restoring GPER-1 expression inhibited by siRNA and G15.
Nothing to Disclose: BSK, YHC, EJL
*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm
See more of: Abstracts - Orals, Featured Poster Presentations, and Posters