Session: SUN 702-709-Obesity: Response to Interventions
Poster Board SUN-703
[Results] Mice (C57BL/6, male) were fed a chow diet (Ch) or HF from 5 to 14 weeks of age. Mice were intraperitoneal injected with 1 mg 5-HT or PBS twice a week in both diet. Soleus muscle and gastrocnemius muscle were obtained from fasted mice in 14 weeks old. 5-HT shifted the profile of muscle fiber type from fast/glycolytic to slow/oxidative in the soleus muscle of HF fed mice. 5-HT increased the oxidative muscle fibers and the NAD+/NADH ratio in gastrocnemius muscle in HF fed mice. Additionally, the intraperitoneal injection of 5-HT elevated the expression of key genes related to energy homeostasis, such as type 2 iodothyronine deiodinase and uncoupling protein 3 in soleus muscle. 5-HT dramatically induced the elevation of mRNA expressions of peroxisome proliferator-activated receptor (PPAR)-γ coactivator 1α (PGC-1α)-b and -c, which were known as novel isoforms of PGC-1α and involved in mitochondrial biogenesis and fatty acid oxidation. Moreover, the pretreatment of several kinds of 5-HT receptor (5HTR) antagonist revealed that 5-HT induced the elevation of expression of PGC-1α-b and -c through the 5HTR2A or 7 in the soleus muscle and 5-HTR7 in the gastrocnemius muscle in mice at 8 weeks of age. Our findings suggest that peripheral 5-HT may contribute to increase the profile of slow/oxidative muscle fiber type and the energy expenditure of skeletal muscle by inducing the gene expressions of PGC-1α-b and -c.
Nothing to Disclose: TN, HW, RS, YN, KS, NS, KW, HA
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