Session: MON 723-757-Renin-Angiotensin-Aldosterone System/Endocrine Hypertension
Bench to Bedside
Poster Board MON-744
Methods: Visceral adipose tissues of PA (n=5), Cushing’s syndrome ( n=5) and non-functioning adrenal adenomas (n=5) were collected for detection of AGT mRNA levels and promoter methylation status. Bisulfite sequencing was used for the determination of methylation status. The effect of DNA methylation on AGT promoter activity was determined using luciferase assay.
Results:The AGT gene expression levels in the adipose tissues of patients with PA were significantly higher than those of CS or NFA (p<0.05). The methylation ratio of promoter region of AGT gene was correlated with the mRNA levels of AGT in the adipose tissues. In vitro study, AGT transcription activities were highly dependent on the promoter methylation status.
Conclusions:Elevated AGT gene expression in the adipose tissues may contribute to the pathogenesis of the metabolic abnormalities in patients with PA. Epigenetic effects of aldosterone on AGT gene should be further clarified.
Nothing to Disclose: YT, MD, TY, MO, MK, FW, YC
*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm
See more of: Abstracts - Orals, Featured Poster Presentations, and Posters