Session: SUN 554-573-Ovarian & Uterine Function I
Poster Board SUN-556
It was thus hypothesized that AGE-RAGE signaling could interfere or attenuate LH action via ERK1/2 activation in human granulosa cells. The effect of human glycated albumin (HGA) either alone or in combination with LH was investigated in human ovarian granulosa cells (KGN) relative to ERK1/2 activation.
Methods: KGN cells were cultured with LH (0.2μg/ml-2μg/ml) or HGA (0.2mg/ml) and LH combined with HGA for 0-2hours. Activation of ERK1/ERK2 was assessed in all conditions by Western immunoblotting.
Results: LH treatment significantly increased p-ERK1/2 levels after 5 to 60 min exposure in human granulosa cells compared to basal levels (P<0.001). The combined treatment of LH and HGA increased p-ERK1/2 levels by 2.5 fold and attenuated ERK1/2 activation over 2 hours.
Conclusions: AGEs presence in the ovary interfere with LH action leading to a sustained activation of ERK1/2 in granulosa cells that is crucial for directing normal follicle development and initiating the ovulation process. Inappropriate activation of ERK1/2 in granulosa cells may block the granulosa cell differentiation pathway and /or impair follicular responses to hormones, leading to ovulation failure that characterizes PCOS.
Nothing to Disclose: ED, CP, SL, EAK, EP, MK
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