Session: SUN 389-408-Signaling Originating from Membrane Receptors
Poster Board SUN-404
We studied FLNA expression in tissue samples from GH-omas (N=10) by western blotting (WB). Moreover, we evaluated the role of FLNA in SSTR2 localization and signaling by gene silencing technique in primary cultured cells from 6 GH-omas. Confocal microscopy was used to detect SSTR2 intracellular localization and WB to evaluate cyclin D1 and SSTR2 expression.
We found that FLNA was expressed at variable levels in GH-oma samples, without any significant correlation with the clinical phenotype. FLNA gene silencing did not induce changes in SSTR2 total levels. Similarly, this manipulation did not affect receptor localization at the plasma membrane. On the contrary, the reduction in cyclin D1 levels induced by the selective SSTR2 agonist (BIM23120) in GH-secreting adenoma cultured cells was abolished in FLNA silenced cells.
These data suggest that FLNA might be implicated in intracellular signalling of SSTR2 by mediating at least some of its antiproliferative effects. In contrast, FLNA does not appear to be necessary for receptor expression and localization at the plasma membrane.
Nothing to Disclose: EP, EG, EV, VC, AL, PB, AS, GM
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