Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: SAT 697-707-Obesity Pathophysiology
Saturday, June 15, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board SAT-699
Elisa Petrangeli*1, Laura de Girolamo2, Deborah Stanco2, Luisa Salvatori3, Giuseppe Coroniti4, Elena Arrigoni5, Stefania Niada5, Lorenzo Principessa4, Linda Ravenna3, Vincenzo Toscano6, Matteo Antonio Russo7 and Anna Teresa Brini5
1Sapienza University of Rome, Rome, Italy, 2IRCCS Istituto Ortopedico Galeazzi, Milan, 3CNR, Institute of Molecular Biology and Pathology, Rome, 4Sapienza University of Rome, Rome, 5University of Milan, Milan, 6Sapienza, UniversitÓ di Roma, Roma, Italy, 7IRCCS San Raffaele Pisana, Rome
Today adipose tissue is not just considered as the primary energy storage organ, but it is also recognized as an important endocrine tissue and an abundant source of mesenchymal stem cells (adipose-derived stem cells, ASCs). Little is known about the effect of obesity on ASCs properties. Since ASCs differentiation and proliferation are determined by their niche, the differences in body fat distribution and microenvironmental features may have several consequences.

We compared ASCs of subcutaneous adipose tissue from 8 class II obese patients (obS-ASCs) and 7 non-obese donors (nS-ASCs) in order to compare their immunophenotype and osteogenic and adipogenic potential. obS-ASCs were also compared to ASCs derived from visceral adipose tissue of the same obese donors (obV-ASCs).

Both subcutaneous and visceral ASCs derived from obese donors showed an impairment of cell proliferation and clonogenic ability. nS-ASCs expressed higher levels of the stem cell marker KLF4 and of the MSCs surface antigens CD54, CD90 and CD34 respect to both ob-ASC populations.

Only obS-ASCs, but not the visceral ones, expressed a significantly lower percentage of CD166 with respect to nS-ASCs. obV-ASCs displayed increased basal levels of alkaline phosphatase (ALP) with respect to obS- and nS-ASC.

When cultured in osteogenic or adipogenic medium, nS-ASC acquired differentiated phenotypes, showing, respectively, strong increase of extracellular calcified matrix and ALP, or intracellular accumulation of lipidic vacuoles, respect to undifferentiated cells. obS-ASC displayed differentiative features, although at less extent, while obV-ASC did not show significative increase of these parameters.

Our results show that obesity has a significant negative impact on the stem cell properties and the differentiation potential of ASCs In particular, obV-ASCs lose most of their stem cell characteristics, including multidifferentiation potential, suggesting an early commitment of these cells. The low plasticity of obV-ASCs towards osteogenic and adipogenic lineages could be due to the altered microenvironment which could steer differentiation of ASCs toward different  lineages. In fact, high levels of both ALP and CD166 occur in a number of cell types such as leukocytes and macrophages. The specific stimuli which can play a key role in ASCs impairment, including the effects of hypoxic status and the obesity-related inflammation, will be further investigated to have a complete picture of the phenomenon.

de Girolamo L, Lucarelli E, Alessandri G, Avanzini MA, Bernardo ME, Biagi E, Brini AT, D'Amico G, Fagioli F, Ferrero I, Locatelli F, Maccario R, Marazzi M, Parolini O, Pessina A, Torre ML. Mesenchymal stem/stromal cells: a new "cells as drugs" paradigm. Efficacy and critical aspects in cell therapy. Curr Pharm Des 2012 [Epub ahead of print]   de Girolamo L, Lopa S, Arrigoni E, Sartori MF, Baruffaldi Preis FW, Brini AT. Human adipose-derived stem cells isolated from young and elderly women: their differentiation potential and scaffold interaction during in vitro osteoblastic differentiation. Cytotherapy 2009; 11:793-803.   Isakson P, Hammarstedt A, Gustafson B, Smith U. Impaired preadipocyte differentiation in human abdominal obesity: role of Wnt, tumor necrosis factor-alpha, and inflammation. Diabetes 2009; 58:1550-7.   Oñate B, Vilahur G, Ferrer-Lorente R, Ybarra J, Díez-Caballero A, Ballesta-López C, Moscatiello F, Herrero J, Badimon L. The subcutaneous adipose tissue reservoir of functionally active stem cells is reduced in obese patients. FASEB J 2012; 26:4327-36   Roldan M, Macias-Gonzalez M, Garcia R, Tinahones FJ, Martin M. Obesity short-circuits stemness gene network in human adipose multipotent stem cells. FASEB J 2011; 25:4111-26.   Baglioni S, Francalanci M, Squecco R, Lombardi A, Cantini G, Angeli R, Gelmini S, Guasti D, Benvenuti S, Annunziato F, Bani D, Liotta F, Francini F, Perigli G, Serio M, Luconi M. Characterization of human adult stem-cell populations isolated from visceral and subcutaneous adipose tissue. FASEB J 2009; 23:3494-505   Ye J, Gimble JM. Regulation of stem cell differentiation in adipose tissue by chronic inflammation. Clin Exp Pharmacol Physiol 2011; 38:872-8.

Nothing to Disclose: EP, LD, DS, LS, GC, EA, SN, LP, LR, VT, MAR, ATB

*Please take note of The Endocrine Society's News Embargo Policy at

Sources of Research Support: This study was partially supported by the Italian Ministry of Health (Ricerca Finalizzata 2007, Progetto Ordinario, RF-IOG-656853).