Increased TNF- alpha; and IL-6 and decreased adiponectin with oral supplementation of algae DHA oil in mice with type 2 diabetes mellitus. "Meta-inflammatory" effect?

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: SUN 780-806-Determinants of Insulin Resistance & Associated Metabolic Disturbances
Basic/Translational
Sunday, June 16, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board SUN-804
Rosana Quezado1, Miguel Nasser Hissa*2, Paulo Roberto Leitão Vasconcelos3, Arthur Alcantara Lima1, Carolina Medeiros da Frota Ribeiro1 and Arnaldo Ribeiro Costa-Lima4
1Scholl of Medicine - Federal University of Ceara, Fortaleza, Brazil, 2Clinica de Metabolismo endocrino, Fortaleza, CE, Brazil, 3Medical School -Federal University of Ceara, Fortaleza, Brazil, 4Federal University of Ceara Brazil, Fortaleza-Ceara, Brazil
Type 2 diabetes mellitus (T2DM), a multifactorial, heterogenic disease, results from associated genetic susceptibility to environmental factors, especially sedentary lifestyle and a diet rich in saturated fats, and obesity. It is characterized by insulin resistance (IR) and by a decrease of the secretion of this hormone. Moderate and chronic inflammation in dysfunctional white adipose tissue, called "meta-inflammation," seems to be the link between obesity, IR and T2DM. The role of adipokines produced by adipose tissue in these diseases has been investigated.

Objetive: to verify whether oral supplementation (OS) of oil blend (MXO) with high relation of omega 9 / omega 6 (ω9/ω6) and low relation of omega 6 / omega 3 (ω6/ω3), from different sources of ω3, interferes with adipokines plasma of mice with T2DM.

Methods: After fed ad libitum with AIN-93G diet until they become adult, Swiss mice (SWM) males received during eleven weeks, an adapted AIN-93HA and hyperlipidic diet, to induce T2DM which were confirmed in 90% of them. Maintained the AIN-93HA diet, the SWM with T2DM received, in groups, during seven days, MXO OS: GA: water (null control) GB: MXO [ω9: ω3 0.4:1; ω6: ω3 8: 1 (neutral control)]; GC: MXO [ω9: 3.7:1 ω3, ω6: ω3-ALA 1.4:1]; GD: MXO [ω9: 3.7:1 ω3, ω6: ω3-EPA + DHA from fish 1.4:1]; GE: MXO [ω9: ω3 3.7:1; ω6: ω3, DHA from algae 1.4:1]. Plasma insulin and adipokines like tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), monocyte activating factor (MCP-1), resistin (RES), leptin (LEP),  inhibitor of plasminogen activator 1 (PAI-1) and adiponectin (AdipoQ) were analysed in duplicate by immunotest ensay (LuminextmCorporation’s xMAP (Multiple Analyte Profiling, x= variable). The interleukin-1 beta (IL-1β) was  analysed by ELISA.

Results:It was found a statistically significant difference between adipokines group GE (ω3-DHA from algae), and the other groups, concerning an increased IL-6 compared to GC and GD (p: 0,026); increase of TNF- α in relation to groups GB (p: 0,040), GC (p: 0,002) and GD (p: 0,005), and AdipoQ decrease compared to GB (p: 0,043), as well as RES between GC (ω3-ALA) and GD (ω3-EPA + DHA) (p: 0,041). There was no statistically significant difference in any of the variables between control groups.

Conclusions: The experimental group supplemented with SWM MXO 4 containing algae DHA with relation ω6: ω3: 1.4:1 and ω9: ω6: 3.7:1 showed a significant increase of inflammatory adipokines (TNF-α and IL-6) and decreased otherwise (AdipoQ), an anti-inflammatory adipokine. The continuity of a high-fat-diet, high in saturated fat, may have compromised the effectiveness of supplementation MXO rich in ω3 and ω9.

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Nothing to Disclose: RQ, MNH, PRLV, AAL, CMDFR, ARC

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm

Sources of Research Support: The Study was supported by National Council for Research and Development (CNPq, Brazilian Ministry of Science and Technology) through a Master Graduate Program (stricto sensu) in Medical Sciences