Session: SUN 780-806-Determinants of Insulin Resistance & Associated Metabolic Disturbances
Poster Board SUN-804
Objetive: to verify whether oral supplementation (OS) of oil blend (MXO) with high relation of omega 9 / omega 6 (ω9/ω6) and low relation of omega 6 / omega 3 (ω6/ω3), from different sources of ω3, interferes with adipokines plasma of mice with T2DM.
Methods: After fed ad libitum with AIN-93G diet until they become adult, Swiss mice (SWM) males received during eleven weeks, an adapted AIN-93HA and hyperlipidic diet, to induce T2DM which were confirmed in 90% of them. Maintained the AIN-93HA diet, the SWM with T2DM received, in groups, during seven days, MXO OS: GA: water (null control) GB: MXO [ω9: ω3 0.4:1; ω6: ω3 8: 1 (neutral control)]; GC: MXO [ω9: 3.7:1 ω3, ω6: ω3-ALA 1.4:1]; GD: MXO [ω9: 3.7:1 ω3, ω6: ω3-EPA + DHA from fish 1.4:1]; GE: MXO [ω9: ω3 3.7:1; ω6: ω3, DHA from algae 1.4:1]. Plasma insulin and adipokines like tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), monocyte activating factor (MCP-1), resistin (RES), leptin (LEP), inhibitor of plasminogen activator 1 (PAI-1) and adiponectin (AdipoQ) were analysed in duplicate by immunotest ensay (LuminextmCorporation’s xMAP (Multiple Analyte Profiling, x= variable). The interleukin-1 beta (IL-1β) was analysed by ELISA.
Results:It was found a statistically significant difference between adipokines group GE (ω3-DHA from algae), and the other groups, concerning an increased IL-6 compared to GC and GD (p: 0,026); increase of TNF- α in relation to groups GB (p: 0,040), GC (p: 0,002) and GD (p: 0,005), and AdipoQ decrease compared to GB (p: 0,043), as well as RES between GC (ω3-ALA) and GD (ω3-EPA + DHA) (p: 0,041). There was no statistically significant difference in any of the variables between control groups.
Conclusions: The experimental group supplemented with SWM MXO 4 containing algae DHA with relation ω6: ω3: 1.4:1 and ω9: ω6: 3.7:1 showed a significant increase of inflammatory adipokines (TNF-α and IL-6) and decreased otherwise (AdipoQ), an anti-inflammatory adipokine. The continuity of a high-fat-diet, high in saturated fat, may have compromised the effectiveness of supplementation MXO rich in ω3 and ω9.
Nothing to Disclose: RQ, MNH, PRLV, AAL, CMDFR, ARC
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