Session: SUN 702-709-Obesity: Response to Interventions
Poster Board SUN-704
Methods: Male C57BL/6J mice (12 weeks old) on high fat/high sucrose (HF/HS) diet received bovine GH (5 µg/g body weight) once daily for 6 weeks. Fat distribution (visceral and subcutaneous) was evaluated with X-ray computed tomography (CT) every two weeks and an intra-peritoneal glucose tolerance test (IPGTT) was performed during the last week of experiment. Gene expressions in epididymal white adipose tissue (eWAT) were quantified by real-time RT-PCR. TBARS, a byproduct of lipid peroxidation induced by oxidative stress, in eWAT were measured. 3T3-L1 adipocytes were treated with GH (5-500ng/mL) in the absence or presence of H2O2 (200µM) for 24 hours and the genes including adiponectin (APN) were analyzed by real-time RT-PCR.
Results: No difference in body weight was observed between the two groups of DIO mice (GH and control). However, CT analyses revealed that GH significantly decreased not only visceral fat mass but also subcutaneous one. In accord with these observations, GH treatment decreased the gross weights of eWAT and subcutaneous WAT (sWAT) while increasing those of skeletal muscles. In addition, GH treatment ameliorated the impaired glucose metabolism as assessed by fasting blood glucose, glucose response, and plasma insulin levels in IPGTT. In eWAT, a key adipose tissue in DIO metabolism, the gene expression of APN and IL-10, anti-inflammatory adipocytokines, were raised by GH treatment. Furthermore, TBARS in eWAT were significantly decreased after GH treatment accompanied by the reduced gene expression of glutathione peroxidase, an anti-oxidative enzyme in the same tissue. In the cultured 3T3-L1 adipocytes, addition of GH in media as low as 5 ng/mL enhanced or recovered the lowered gene expression of APN under oxidative stress with H2O2.
Conclusions: GH ameliorates the impaired glucose metabolism in DIO mice by regulating not only adipose mass but also its condition such as inflammation and oxidative stress on visceral fat.
Nothing to Disclose: MF, YO, SI, HK, MO, SM
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