Quantitative Analysis of Gene Expression in the Brain of Transgenic bGH and Wild Type Mice

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: MON 142-166-Hypothalamus-Pituitary Development & Biology
Monday, June 17, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board MON-145
Amrita Basu* and John Joseph Kopchick
Ohio University, Athens, OH
Growth hormone (GH) and GH receptor (GHR) have been experimentally found to be present in various regions of the mammalian brain (e.g. cerebral cortex, hippocampus, hypothalamus, amygdala, etc.). Therefore, GH induced signaling is expected to be relevant in development and functions of the central nervous system. Changes in GH signaling status as observed in acromegaly (increased GH signaling), dwarfism (reduced GH signaling) and Laron Syndrome (absence of GH signaling due to non-functional GHR) have been correlated with changes in neuronal cell proliferation, myelination, post-traumatic neuroprotection, cognition, memory and aging, in human and animal models. Also, Alzheimer’s disease (AD) has been associated with perturbation in insulin/insulin-like growth factor 1 (IGF-1) signaling and glucose metabolism in the brain. GH, a major regulator of IGF-1 production and a diabetogenic hormone due to its anti-insulin action, could therefore be of potential significance in modulating learning and memory in normal and/or disease conditions. Comprehensive information about the molecular mechanism of GH action in the brain is not yet well understood. The present study aims at quantitative estimation of expression of six genes (GH, GHR, insulin, insulin receptor, IGF-1 and IGF-1 receptor) in mouse brain. Total RNA was isolated from four different brain areas (cerebral cortex, cerebellum, hippocampus and hypothalamus) of 6 month old, male, bovine GH (bGH) transgenic mice and wild type (WT) controls (n=7), followed by cDNA synthesis. Quantitative real –time polymerase chain reaction (qRTPCR) will be used to quantify fold changes in expression of the genes of interest. Locomotor activity, spatial learning and memory retention abilities will be compared among bGH and WT mice using a Barnes Maze study. The study will benefit in understanding of molecular and behavioral effect of GH in the brain, elucidation of possible correlation between GH/IGF-1 levels with AD and identification of potential therapeutic targets for neuronal diseases.

Nothing to Disclose: AB, JJK

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm

Sources of Research Support: This work was supported in part by the State of Ohio’s Eminent Scholar Program that includes a gift from Milton and Lawrence Goll, by the AMVETS, by the Diabetes Institute at Ohio University, and by NIH grants DK083729, AG031736.