FP38-5 ASSOCIATION BETWEEN CONGENITAL CRYPTORCHIDISM AND LEVELS OF PERSISTENT ORGANIC POLLUTANTS IN SUBCUTANEOUS FAT

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: FP38-Physiological Impacts of Endocrine Disrupting Chemicals
Basic/Translational
Monday, June 17, 2013: 10:45 AM-11:15 AM
Presentation Start Time: 11:05 AM
Room 256 (Moscone Center)

Poster Board MON-339
Jaakko J Koskenniemi1, Helena E Virtanen1, Hannu Kiviranta2, Katharina M Main3, Niels Erik Skakkebaek4 and Jorma Toppari*1
1Univ of Turku, Turku, Finland, 2National Institute of Health and Welfare, Kuopio, Finland, 3Rigshospitalet, University of Copenhagen, Copenhagen, Denmark, 4University of Copenhagen, Copenhagen, Denmark
Congenital cryptorchidism, i.e. the absence of the testis in the scrotum at birth, is a common genital malformation among the newborn boys. The evidence from epidemiological, wildlife and animal studies suggests that the exposure to Endocrine Disrupting Chemicals (EDCs) during fetal development might play a role in its pathogenesis. However, associations between congenital cryptorchidism and exposure to EDCs have been observed in only few human studies.  Persistent Organic Pollutants (POPs) such as polychlorinated biphenyls (PCBs), polychlorinated dibenzo-p-dioxins and furans (PCDD/Fs) and polybrominated diphenyl ethers (PBDEs) are chemicals that bioaccumulate to the tissue lipid and to the top of the food chain. In the intrauterine milieu, the child is exposed to POPs through placenta and in postnatal life predominantly via breast milk. Later on, a significant source of the PBDEs is indoor dust, whereas exposure to PCDD/Fs and PCBs comes mainly from the ingestion of fatty foods. The aim of this study was to find out whether exposure to PCDD/Fs, PCBs, or PBDEs is associated with congenital cryptorchidism.

The study (approved by the ethics committee) included fat biopsies from 30 volunteer Finnish boys that were referred for orchiopexy (cases) and 29 boys that were operated for inguinal hernia, abdominal hernia or hydrocele (controls) at Turku University Hospital in 2002 - 2006. During the operation, a subcutaneous fat biopsy was taken. In addition, the parents filled in a questionnaire concerning the breastfeeding of the child. Samples were analyzed for 37 PCBs, 17 PCDD/Fs and 14 PBDEs.

After the data was adjusted for postnatal confounders such as the age of the mother, total period of exclusive breastfeeding, age when the breastfeeding was discontinued and age when the child was operated, cryptorchid cases had significantly higher ∑PCDD/F (median case vs control 99.2 pg/g vs 63.2 pg/g, p=0.02) and the WHO-TEq of the 17 dioxins and 12 dioxin-like PCBs i.e. total-TEq (6.7 pg/g vs 4.1 pg/g, p=0.002) than controls. No significant  association was found between congenital cryptorchidism and ∑PBDEs (6.6 ng/g vs 4.9 ng/g, p=0.53) or ∑PCB (68.5 ng/g vs 79.5 ng/g, p=0.06) in adipose tissue.

In conclusion, pre- and postnatal exposure to dioxin-like PCBs and PCDD/Fs might be associated with congenital cryptorchidism.

Nothing to Disclose: JJK, HEV, HK, KMM, NES, JT

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm

Sources of Research Support: Supported by: Academy of Finland, Sigrid Juselius Foundation, EU Environment.