Perilipin 3 expression and adipose tissue lipolysis is increased after 16 weeks of exercise training in women with polycystic ovary syndrome

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: SUN 678-689-Adipocyte Biology
Sunday, June 16, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board SUN-681
Jeffrey D Covington*1, Sudip Bajpeyi2, Yourka D Tchoukalova1, Cedric Moro3, Philip J Ebenezer1, David H Burk1, Eric Ravussin4 and Leanne M Redman4
1Pennington Biomedical Research Center, Baton Rouge, LA, 2University of Texas, El Paso, El Paso, TX, 3Hannover Medical School, Hannover, Germany, 4Pennington Biomed Res Ctr, Baton Rouge, LA
Polycystic Ovary Syndrome (PCOS) is an endocrine condition associated with reduced adipose tissue lipolytic activity, a trait which can be rescued by aerobic exercise.  To understand the potential mechanism underlying this phenomenon, we recruited 8 women with PCOS and 8 women with normal cycles, matched for BMI and age. Abdominal subcutaneous adipose tissue was collected by biopsy to measure Perilipin 3 (PLIN3), a member of the perilipin family of proteins (PAT proteins), using Real Time PCR. The expression of PLIN3 was 90% lower (p<0.0001) in women with PCOS when compared to normally menstruating controls, and was increased by 50% (p=0.04) following exercise training. Primary adipose-derived stromal/stem cell cultures were also established before and after 16 weeks of aerobic exercise training in the women with PCOS only. In primary adipose cultures, PLIN3 protein expression (by western blot) was virtually nonexistent in PCOS women prior to exercise; however in response to exercise training, PLIN3 expression significantly increased to levels similar to normal controls. Furthermore, exercise training increased in vitro adipose oleate oxidation and glycerol secretion by 20% (p=0.03) and 40% (p=0.04) respectively, along with a 50% reduction in triglyceride content by (p=0.01) and a drastic reduction in lipid droplet size. These findings suggest that PLIN3 may be an important regulator of lipolysis and lipid oxidation in women with PCOS.

Nothing to Disclose: JDC, SB, YDT, CM, PJE, DHB, ER, LMR

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