OR53-3 Simultaneous Deletion of the Transcription Factors GATA4 and GATA6 Results in Adrenal Agenesis in Mice

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: OR53-Transcriptional Regulators & Epigenetic Control
Basic/Translational
Tuesday, June 18, 2013: 9:15 AM-10:45 AM
Presentation Start Time: 9:45 AM
Room 133 (Moscone Center)
Sergei G Tevosian*, Maria Beatriz Padua, Shawna C Fox and Deb Morse
University of Florida, Gainesville, FL
The main function of the adrenal gland is hormone synthesis and secretion to support the vital functions. The murine adrenal glands consist of an outer cortex and an inner medulla. The adrenal cortex produces a complex array of steroid hormones, whereas the medulla is part of the sympathetic nervous system and produces the catecholamines epinephrine and norepinephrine. In the mouse, GATA4 and GATA6 transcription factors are co-expressed in several embryonic tissues, including the adrenal cortex. To investigate the roles of Gata4 and Gata6 in mouse adrenal development, we conditionally inactivated these genes in the adrenal cortex. We report here that mice with double deletions of the Gata4; Gata6 genes lack identifiable adrenal glands. Single inactivation of Gata6 gene alone notably reduced adrenal size and corticosterone production in the adult animals. In contrast, a single allele of Gata6 in Gata4 conditional knockout animals was sufficient for normal adrenal organogenesis. Our results reveal a requirement for GATA factors in the adrenal development and maintenance, and define a new mouse model to characterize the transcriptional network controlling adrenocortical cell fates.

Nothing to Disclose: SGT, MBP, SCF, DM

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