DIETARY N-METHYLSEROTONIN REGULATES SKIN TEMPERATURE IN A FEMALE RAT MODEL OF MENOPAUSE-RELATED HOT FLASH

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: MON 515-547-Female Reproductive Endocrinology
Basic/Translational
Monday, June 17, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board MON-538
Michael James Weiser*, Augusta E Garrison and Christopher M Butt
DSM, Boulder, CO
Clinical evidence suggests that supplementation with black cohosh (Cimicifuga racemosa) relieves symptoms of menopause, but the data are mixed. N-methylserotonin (NMS) is a minor component of black cohosh and is a selective agonist of a serotonin (5-HT) receptor subtype that is involved in thermoregulation (5-HT7).  NMS also has inhibitory activity at the serotonin reuptake transporter [SERT; Powell et al. (2008) J Agric Food Chem 56:11718-26]. These findings implicate NMS as an active component of black cohosh that may relieve hot flash and mood-related symptoms of menopause, but the in vivo effects and effective dose(s) of NMS are currently unknown. In this study we sought to determine the effects of dietary supplementation with NMS on induced hot flash and mood in female rats. Ovariectomized (OVX) female rats were fed diets that contained different levels of NMS or were given estradiol implants. The animals were then tested for locomotor activity in the open field, anxiety-like behaviors on the elevated plus maze, and depression-like behaviors with the forced swim test. Hot flashes were subsequently induced with intravenous calcitonin gene-related peptide, and skin temperature was monitored. The results indicated that NMS supplementation did not affect OVX-induced weight gain, uterine growth, or mood-related behaviors. However, NMS supplementation did significantly lower baseline skin temperature and blunted the hot flash response in a manner similar to that observed in estradiol implanted animals. These in vivo findings support NMS as an active component of black cohosh that could decrease menopausal hot flashes without the potential risks associated with hormone replacement therapy or phytoestrogen use.

Disclosure: MJW: Employee, DSM. AEG: Employee, DSM. CMB: Employee, DSM.

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm