Impact of Chlopyrifos and Methanol on Aquatic Snail Reproduction

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: SUN 366-382-Physiological Impacts of Endocrine Disrupting Chemicals
Basic/Translational
Sunday, June 16, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board SUN-379
Terri L Provost*1, Courtney Healy2 and Stephanie Calenzo2
1Utica College, Utica, NY, 2Utica College
The organophosphate pesticide Chlorpyrifos is used extensively in agriculture and has been shown to have adverse effects on the nervous system, reproduction and growth. This chemical frequently appears in farm run-off, a common habitat for the aquatic snail Helimosa trivolvis. We exposed H. trivolvis to concentrations of Chlorpyrifos commonly found in natural habitats to investigate reproductive success and stress. Sixty pairs of snails were treated with one of four Chlorpyrifos concentrations (0.025%, 0.05%, 0.25%, or 0.5%), vehicle control of methanol (200µl/200ml) or control (aged water) for 30 days. Reproductive success was determined by egg mass quantity and day of egg mass production post Chlorpyrifos exposure. Reproductive physiology was determined by measuring estrogen and testosterone while stress response was measured by corticosterone. Hormones were measured radiometrically in snail body homogenate. Treatment with 0.25% and 0.5% Chlorpyrifos caused death rates of 85% and 90%, respectively while death rates in lower pesticide concentrations were similar to control snails. The reproductive rates, as measured by egg mass production, were significantly lower in the vehicle control and all Chlorpyrifos treatments when compared with control rates. Estrogen was significantly depressed in vehicle control and all Chlorpyrifos treatments in a dose dependent manner when compared with controls, while testosterone and corticosterone were unaffected. These data indicate that methanol had no impact on death but negatively impacted reproductive success. Results made determining the impact of Chlorpyrifos on reproduction difficult. Future research will clarify the impact that Chlorpyrifos has on reproductive success using safe levels of methanol.

Nothing to Disclose: TLP, CH, SC

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