Changes in subclinical inflammatory markers observed during puberty are not related to insulin levels in a healthy pediatric population

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: SUN 758-779-Cardiometabolic Risk & Vascular Biology
Clinical
Sunday, June 16, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board SUN-777
Hernan Garcia*1, Clarita Ferrada1, Alejandro Martinez1, Marlene Aglony2, Carolina Avalos3, Carolina Andrea Loureiro3, Lilian Bolte3, Cristian A Carvajal2, Carmen Campino2, Rodrigo Bancalari2 and Carlos Fardella4
1Universidad Catolica de Chile, Santiago, 2Pontificia Universidad Catolica de Chile, Santiago, Chile, 3Pontificia Universidad Católica de Chile, Santiago, Chile, 4Universidad Catolica de Chile
Introduction

Puberty is associated with a physiologic inflammatory condition, in part due to the reduced insulin sensitivity observed at this age. The inflammatory markers profile, and their relationship with insulin levels has not been established in healthy children.

Objectives

To compare  inflammatory markers (IM) profile between pubertal vs prepubertal normal children and their correlation with Insulin levels.

Subjects y Methods

113 healthy children with normal weight and blood pressure  of 10.59 ± 2.82 years old, 55.8% female, 58.4% pubertal were studied. Height and pubertal staging were registered. A fasting blood sample to determinate Alpha tumoral necrosis factor (TNF α), Interleukin 6 (IL6) and 8(IL8), plasminogen activator inhibitor-I (PAI I), Adiponectin (Ad), Ultrasensitive reactive C protein (usRCP) and Insulin (Ins) levels was obtained . Differences between these parameters by age, gender, puberty  and their correlation with Ins levels were analyzed.

RESULTS

PAI-I  (median and intequartile range) was 8,51 ( 5,66-15,68) and  14,72 (10,22-22,56) ng/ml in prepubertal and pubertal children respectively (P:0,0006). Ad  was 14,2  (12,6-18,2) vs. 12,65 (9,6-15,3) ug/ml in prepubertal and pubertal children  respectively (p: 0,015). Ad differences were observed only in  boys.

No significant differences were observed in  TNF α, IL6, IL8,  and usRCP by age, gender or pubertal state.  

No significant correlation  were  demonstrated between    TNF α, IL6, IL8,  usRCP, and Ad with  BMI. 

Levels obtained for the inflammatory parameters were:  TNF α: 17,32 pg/ml (12,6-24,23) , IL6: 11,85 pg/ml (8,33-14,6), IL8: 19,45 pg/ml (16,04-28,21),  usRCP: 0,29 mg/l (0,2-0,77). Ins  was 8,1 u/ml (6,2-9) and was not correlated with age, puberty, BMI, PAI I or Ad.

 CONCLUSIONS

Inflammatory state is higher during puberty according to  PAI-I and Adiponectin levels independently of insulin  concentration  in normal children .

This suggests that these could be  the most sensitive markers for estimate endothelial dysfunction in adolescents.

Values obtained in this normal population, may be useful as a reference in clinical practice.

Supported by Chilean grants: FONDEF D08i1087, FONDEF IDeA CA12i10150, FONDECYT 1100356 & 1130427 & IMII P09/016-F.

Nothing to Disclose: HG, CF, AM, MA, CA, CAL, LB, CAC, CC, RB, CF

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm

Sources of Research Support: Supported by Chilean grants: FONDEF D08i1087, FONDEF IDeA CA12i10150, FONDECYT 1100356 & 1130427 & IMII P09/016-F. CAC is PhD fellow from Comision Nacional y Científica de Chile (CONICYT).