Session: SUN 678-689-Adipocyte Biology
Poster Board SUN-680
Blomquist, C.1, Chorell, E.1, Ryberg, M.1, Sandberg, S. 2, Mellberg, C. 1, Lindahl, B.2, Larsson, C. 3,Olivecrona, G.4 Olsson, T.1
1. Public health and clinical medicine, Department for medicine, Umeå University
2. Public health and clinical medicine, Department for environmental medicine, Umeå University
3. Department of Food and Nutrition, and Sport Science, University of Gothenburg
4. Medical Biosciences, Umeå University
Disclosures: Nothing to disclose
In postmenopausal women, there is a tendency for weight gain to occur in the abdominal region and the magnitude of the increase in abdominal adiposity correlates with obesity-related risk factors such as ectopic fat distribution, hyperlipidemia, and type 2 diabetes. It is therefore important to find a diet that promotes abdominal weight loss and reduces ectopic fat distribution and metabolic complication in postmenopausal women.
71 overweight/obese postmenopausal women were randomized to a Paleolithic type diet high in protein (30E%), mono- and polyunsaturated fatty acids and low in carbohydrates (30E%) or a diet based on Nordic Nutrition Recommendations (NNR) high in carbohydrates (55-60E%) and with a lower intake of protein (15E%) and fat (25-30E%) for 24 months. Anthropometric data, blood samples and abdominal subcutaneous fat biopsies were collected at baseline, 6 and 24 months. We analyzed genes regulating fat metabolism in adipose tissue samples taken at baseline and six months, where the largest overall weight loss occurred. Lipoprotein lipase (LPL) enzyme activity and mass were quantified at all time points.
There were a significant decrease in BMI and waist in both diet groups during the first 6 months with weight maintenance at 24 months. The significant decrease in diglyceride acyltransferase (DGAT) mRNA levels was only seen during the first 6 months in the Paleolithic group. Throughout the study period a decrease in LPL mass and activity was seen for both diet groups. Within the Paleolithic group, the observed weight loss was positively associated with LPL mass/activity as well as gene expression levels (mRNA) for enzyme and transporters in fat metabolism such as DGAT, fatty acid binding protein 4 and CD 36.
To conclude, LPL and DGAT are important regulatory steps in adipose tissue fatty acid (FA) uptake and storage, where LPL activity directs regional FA distribution and DGAT regulates the final step in triglyceride synthesis. The downregulation of these two key factors in fat disposal may contribute to a beneficial abdominal fat mass reduction on a longterm basis among postmenopausal women.
Nothing to Disclose: CB, EC, MR, SS, CM, BL, CL, GO, TO
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