Session: MON 561-585-Ovarian & Uterine Function II
Poster Board MON-573
Objective: we hypothesized that the AMH and AMHR2 polymorphisms are associated with serum estradiol, FSH and AMH; controlled ovarian hyperstimulation response and assisted reproduction outcomes.
Methods: Case-control study comprising 136 infertile women (n=35 with Idiopathic infertility, n=39 with tubal obstruction, n=62 women with male factor involved). Detection of T146G and A-482G polymorphisms of the genes AMH and AMHR2 were performed using TaqMan methodology by real time PCR. The results were analyzed statically and a p-value <0.05 was considered significant. The measurement of estradiol and FSH serum levels was performed by Elisa assay. An AMH serum level was measured using the Elisa assay AMH Gen II.
Results: Statistical analysis revealed that T146G and A-482G polymorphisms were not significantly when compared to serum FSH (p=0,387; p=0,363), estradiol (p=0,208; p=0,599) and AMH (p=0,946; p=0517), respectively. The correlation between the polymorphisms of the AMH and AMHR2genes and the results of controlled ovarian hyperstimulation also did not show a significant difference (p=0,165 e p=0,644), thus the genotypes do not influence in the ovarian response. Even when we separated by infertility factor and compared with embryo parameters we did not find a significant difference. However if we compared the number of cycles performed with infertility factor, patients with idiopathic infertility perform fewer cycles than patients with male factor or tubal obstruction, p=0,001.
Conclusion: It has been suggested that polymorphisms in AMH and AMHR2 genes may influence hormone function in folliculogenesis and cause the arrest of follicular growth and so, leads to decreased of ovarian reserve. In the present study, the polymorphisms T146G and A-482G of the AMH and AMHR2genes were not associated with the FSH, AMH and estradiol levels or with the assisted reproductive outcomes.
Nothing to Disclose: CP, CT, EC, VC, DC, CPB, BB
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