The evaluation of AMH and AMHR2 genes polymorphisms in infertile women and the correlation with assisted reproduction outcomes

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: MON 561-585-Ovarian & Uterine Function II
Basic/Clinical
Monday, June 17, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board MON-573
Carla Peluso1, Camila Trevisan1, Emerson Cordts1, Viviane Cavalcanti1, Denise Christofolini1, Caio Parente Barbosa1 and Bianca Bianco*2
1Faculdade de Medicina do ABC, Santo Andre, Brazil, 2Faculdade de Medicina do ABC, Sao Paulo, Brazil
Introduction: Recent studies have demonstrated that serum AMH levels reflect the size of the primordial follicle pool. Studies in Amhnull mice showed that, in the absence of AMH, follicles are recruited at a faster rate, and are more sensitive to FSH. So, AMH can be a marker of ovarian reserve and can predict the ovarian response.

Objective: we hypothesized that the AMH and AMHR2 polymorphisms are associated with serum estradiol, FSH and AMH; controlled ovarian hyperstimulation response and assisted reproduction outcomes.

Methods: Case-control study comprising 136 infertile women (n=35 with Idiopathic infertility, n=39 with tubal obstruction, n=62 women with male factor involved). Detection of T146G and A-482G polymorphisms of the genes AMH and AMHR2 were performed using TaqMan methodology by real time PCR. The results were analyzed statically and a p-value <0.05 was considered significant. The measurement of estradiol and FSH serum levels was performed by Elisa assay. An AMH serum level was measured using the Elisa assay AMH Gen II.

Results: Statistical analysis revealed that T146G and A-482G polymorphisms were not significantly when compared to serum FSH (p=0,387; p=0,363), estradiol (p=0,208; p=0,599) and AMH (p=0,946; p=0517), respectively. The correlation between the polymorphisms of the AMH and AMHR2genes and the results of controlled ovarian hyperstimulation also did not show a significant difference (p=0,165 e p=0,644), thus the genotypes do not influence in the ovarian response. Even when we separated by infertility factor and compared with embryo parameters we did not find a significant difference. However if we compared the number of cycles performed with infertility factor, patients with idiopathic infertility perform fewer cycles than patients with male factor or tubal obstruction, p=0,001.

Conclusion: It has been suggested that polymorphisms in AMH and AMHR2 genes may influence hormone function in folliculogenesis and cause the arrest of follicular growth and so, leads to decreased of ovarian reserve. In the present study, the polymorphisms T146G and A-482G of the AMH and AMHR2genes were not associated with the FSH, AMH and estradiol levels or with the assisted reproductive outcomes.

Nothing to Disclose: CP, CT, EC, VC, DC, CPB, BB

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm

Sources of Research Support: Support: This work was supported by grants from FAPESP (Fundação de Amparo a Pesquisa do Estado de São Paulo) # 2011/08681-1 and #2011/15045-4.