BONE MORPHOGENIC PROTEIN 7 AND SMADs IN POLYCYSTIC OVARY

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: SUN 554-573-Ovarian & Uterine Function I
Basic/Clinical
Sunday, June 16, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board SUN-558
Gloria Barbosa-Sabanero*1, Juan Manuel Malacara Hernandez2, Gustavo Romero Gutierrez3, Myrna Sabanero-Lopez4 and Heidi Cecilia Muņoz-Pedroza5
1Univ de Guanajuato, Leon GTO, Mexico, 2UNIVERSIDAD DE GUANAJUATO, LEON. GTO., 3INSTITUTO MEXICANO DEL SEGURO SOCIAL, LEON.GTO, Mexico, 4UNIVERSIDAD DE GUANAJUATO, GUANAJUATO. GTO., Mexico, 5UNIVERSIDAD DE GUANAJUATO, LEON.GTO, Mexico
Introduction. Polycystic ovary syndrome (PCOS) occurs in 5 to 10% of women and characterized by hirsutism, oligo-anovulation, infertility and polycystic ovaries. In PCOS, the growth of primary follicles is slow. Small antral follicles have an arrest in in its development in the final stages of maturation and are accumulate. These follicles mainly show characteristics associated with atresia and apoptosis in granulosa cells. BMP7 belongs the family of growth factors with action on target proteins through SMAD 1/5/8 cascade, and has been suggested to have an anti-luteolytic and anti-apoptotic effect, with a crucial role in the conversion of androgens to estradiol. This implies that a hyperandrogenic condition such as PCOS dysfunction could be caused by a defect in the BMPs signaling.

Methods. Ovarian wedges were obtained from ten healthy women and ten PCOS patients. Anthropometric data were collected and blood levels of hormones were obtained. Immunodetection and densitometry of BMP7 and SMAD 1/5/8 in control and PCOS women were carried out. In addition, two animal models of PCOS were carried out in rats. One of them with the application of estradiol valerate (i.m. 4 mg/rat of 200 g) and the other with testosterone propionate (i.m. 1.25 mg/neonatal rat). FSH and LH were measured in blood samples. Ovaries were obtained to evaluate BMP7 and SMAD 1/5/8. As primary antibodies was used anti-BMP7 (1:250) and anti-SMAD 1/5/8 (1:500) for human and rat. As secondary antibody was used anti-goat IgG- peroxidase  1:500 to detect human BMP7, 1:3000 for human SMAD 1/5/8; 1:1500 to detect BMP7 and SMAD 1/5/8 in rat.

RESULTS. Patients with PCOS showed an increase of BMP7 and an increase of SMAD 1/5/8 as compared with controls. The same trend was observed in rats treated with estradiol valerate, BMP7 protein was increased and SMAD 1/5/8 protein decreased as comparison with the controls. In rats treated with testosterone propionate we found that both the BMP7 and SMAD 1/5/8 decreased.

CONCLUSIONS. BMP7 and SMAD 1/5/8 proteins may participate in the pathophysiology of SOP. The rat model induced with estradiol valerate showed a more similarity in the changes of BMP7 and SMAD proteins observed in PCOS.

Nothing to Disclose: GB, JMM, GR, MS, HCM

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