Session: SUN 554-573-Ovarian & Uterine Function I
Poster Board SUN-558
Methods. Ovarian wedges were obtained from ten healthy women and ten PCOS patients. Anthropometric data were collected and blood levels of hormones were obtained. Immunodetection and densitometry of BMP7 and SMAD 1/5/8 in control and PCOS women were carried out. In addition, two animal models of PCOS were carried out in rats. One of them with the application of estradiol valerate (i.m. 4 mg/rat of 200 g) and the other with testosterone propionate (i.m. 1.25 mg/neonatal rat). FSH and LH were measured in blood samples. Ovaries were obtained to evaluate BMP7 and SMAD 1/5/8. As primary antibodies was used anti-BMP7 (1:250) and anti-SMAD 1/5/8 (1:500) for human and rat. As secondary antibody was used anti-goat IgG- peroxidase 1:500 to detect human BMP7, 1:3000 for human SMAD 1/5/8; 1:1500 to detect BMP7 and SMAD 1/5/8 in rat.
RESULTS. Patients with PCOS showed an increase of BMP7 and an increase of SMAD 1/5/8 as compared with controls. The same trend was observed in rats treated with estradiol valerate, BMP7 protein was increased and SMAD 1/5/8 protein decreased as comparison with the controls. In rats treated with testosterone propionate we found that both the BMP7 and SMAD 1/5/8 decreased.
CONCLUSIONS. BMP7 and SMAD 1/5/8 proteins may participate in the pathophysiology of SOP. The rat model induced with estradiol valerate showed a more similarity in the changes of BMP7 and SMAD proteins observed in PCOS.
Nothing to Disclose: GB, JMM, GR, MS, HCM
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