Session: MON 561-585-Ovarian & Uterine Function II
Poster Board MON-574
OBJECTIVE: To correlate the C677T and A1298C polymorphisms of the MTHFR gene with estradiol serum level and to assisted reproduction outcomes.
MATERIAL AND METHODS: 142 infertile women infertile women that underwent in vitro fertilization (n=41 tubeperitoneal factor, n=66 male infertility and n=35 idiopathic infertility). All patients were younger than 38 years old, had normal prolactin and TSH serum levels, presence of both ovaries without morphological abnormalities, ovulatory cycle, body mass index ≤30, no previous history of poor ovulatory response, and no evidence of endocrine disorders or endometriosis. Genotyping of C677T and A1298C polymorphisms were performed using TaqMan methodology by real time PCR. The measurement of estradiol was performed by ELISA. The results were analyzed statistically and a p value <0,05 was considered significant.
RESULTS: Considering the controlled ovarian hyperstimulation response 66.9% (95/142) showed good response, 24.6% (35/142) poor response; 5.6% (8/142) hyper response, and 2.8% (4/142) developed ovarian hyperstimulation syndrome. The results of ovarian stimulation, serum estradiol levels and assisted reproduction outcomes (such as number of retrieved oocytes, embryo transferred, good embryos quality and/or pregnancy rate) showed no statistically significant difference when correlated to the MTHFR polymorphisms.
CONCLUSION: Previous studies observed that women underwent assisted reproduction techniques and carriers of the T allele of the MTHFR C677T polymorphism showed reduced ovarian response to recombinant FSH (FSHr). Furthermore, these patients have significantly lower concentrations of estrogen and showed significantly less oocytes retrieved. However, the present study found no statistically significant difference for polymorphisms correlated with serum levels of estradiol, ovarian stimulation and assisted reproduction outcomes.
Nothing to Disclose: CP, BLL, EC, VC, DC, CPB, BB
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