MTHFR gene polymorphisms are not correlated with estradiol serum levels and assisted reproduction outcomes

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: MON 561-585-Ovarian & Uterine Function II
Monday, June 17, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board MON-574
Carla Peluso1, Barbara Lima Lechado1, Emerson Cordts1, Viviane Cavalcanti1, Denise Christofolini1, Caio Parente Barbosa1 and Bianca Bianco*2
1Faculdade de Medicina do ABC, Santo Andre, Brazil, 2Faculdade de Medicina do ABC, Sao Paulo, Brazil
INTRODUCTION: The MTHFR gene involved in folate metabolism plays an important role in homocysteine metabolism. The gene has two polymorphisms that lead to a decreased activity of the MTHFR enzyme. Recent studies indicate that the synthesis of androgens and estrogens also suffer the influence of these polymorphisms in the MTHFR gene. Different concentrations of these hormones in the serum may have negative correlations with the assisted reproduction outcomes as number of oocytes retrieved, implantation rate and abortion.

OBJECTIVE: To correlate the C677T and A1298C polymorphisms of the MTHFR gene with estradiol serum level and to assisted reproduction outcomes.

MATERIAL AND METHODS: 142 infertile women infertile women that underwent in vitro fertilization (n=41 tubeperitoneal factor, n=66 male infertility and n=35 idiopathic infertility). All patients were younger than 38 years old, had normal prolactin and TSH serum levels, presence of both ovaries without morphological abnormalities, ovulatory cycle, body mass index ≤30, no previous history of poor ovulatory response, and no evidence of endocrine disorders or endometriosis. Genotyping of C677T and A1298C polymorphisms were performed using TaqMan methodology by real time PCR. The measurement of estradiol was performed by ELISA. The results were analyzed statistically and a p value <0,05 was considered significant.

RESULTS: Considering the controlled ovarian hyperstimulation response 66.9% (95/142) showed good response, 24.6% (35/142) poor response; 5.6% (8/142) hyper response, and 2.8% (4/142) developed ovarian hyperstimulation syndrome. The results of ovarian stimulation, serum estradiol levels and assisted reproduction outcomes (such as number of retrieved oocytes, embryo transferred, good embryos quality and/or pregnancy rate) showed no statistically significant difference when correlated to the MTHFR polymorphisms.

CONCLUSION: Previous studies observed that women underwent assisted reproduction techniques and carriers of the T allele of the MTHFR C677T polymorphism showed reduced ovarian response to recombinant FSH (FSHr). Furthermore, these patients have significantly lower concentrations of estrogen and showed significantly less oocytes retrieved. However, the present study found no statistically significant difference for polymorphisms correlated with serum levels of estradiol, ovarian stimulation and assisted reproduction outcomes.

Nothing to Disclose: CP, BLL, EC, VC, DC, CPB, BB

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Sources of Research Support: NEPAS/FMABC