The receptor CD44 is associated with insulin resistance in Caucasian subjects

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: SUN 649-677-Adipocyte Biology
Sunday, June 16, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board SUN-668
Li Fen Liu*1, Keiichi Kodama2, Atul J Butte2, Tracey L McLaughlin3 and Ke Wei2
1Stanford University, Palo Alto, CA, 2Stanford University, Palo Alto, 3Stanford Univ. School of Medicine, Stanford, CA
Objective: Pro-inflammatory immune cells infiltration in human adipose tissues is associated with the development of insulin resistance. Previously Butte and Kodama identified the receptor CD44 as the top candidate gene linked to type 2 diabetes, and reported that CD44 deficiency in diabetic mice improves insulin resistance. Also serum CD44 levels were positively correlated HOMA-IR and HbA1c in Japanese. Association of CD44 in human adipose tissue has not been evaluated with regard to insulin resistance, nor have non-Japanese populations been studied. Methods:  Subjects included 41 healthy, overweight/moderately obese adults with body mass index (BMI) 25–36.9 kg/m2 who met predetermined criteria for being insulin resistant (IR) or insulin sensitive (IS) based on the modified insulin suppression test. Fasting serum human sCD44std (standard soluble CD44) was measured in a total of 41 serum samples (n=21, IS, n=20 IR) by using a quantitative enzyme-linked immunosorbent assay (ELISA).  Expression level of CD44 gene in abdominal subcutaneous adipose tissues was determined by using Quantitative real-time polymerase chain reaction, qRT-PCR. Results: There was no statistically significant difference in BMI between IS and IR individuals. Serum CD44 levels were significantly higher in the IR group (265 ± 94 vs 326 ± 85.6 ng/mL, IS  vs. IR, respectively, P =0.03) CD44 gene expression in adipose tissues was significantly higher in the IR group (P < 0.05). Conclusions:  Our data add evidence for CD44 being a molecular target in adipose tissue inflammation associated with insulin resistance in Caucasians. Further study is needed to elucidate the mechanism by which inflammation and insulin resistance are associated with CD44 in adipose tissue.

Nothing to Disclose: LFL, KK, AJB, TLM, KW

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