Comparative Gene Array Analysis of Human Deep Neck and Subcutaneous Adipose Tissue Progenitor Cells

Program: Abstracts - Orals, Poster Preview Presentations, and Posters
Session: SUN 0875-0890-Adipocyte Biology-Clinical studies
Sunday, June 22, 2014: 1:00 PM-3:00 PM
Hall F (McCormick Place West Building)

Poster Board SUN-0888
Daniel Tews1, Verena Schwar1, Theresia Weber1, Marc Scheithauer1, Thomas Barth1, Peter Möller1, Tobias Fromme2, Martin Klingenspor2, Pamela Fischer-Posovszky1 and Martin Wabitsch1
1University Medical Center Ulm, 2Technical University Munich
Introduction Studies in animal models revealed that brown and white adipocytes derive from different progenitor cells. Molecular characteristics of these cells have not been investigated in detail in humans. Here we sought to identify novel markers of human brown adipocyte progenitor cells.

Methods Paired deep neck and subcutaneous adipose tissue samples from n=52 subjects were taken and subdivided for analysis by immunohistochemistry (n=26) and quantitative real-time PCR (n=14), respectively. Progenitor cells were isolated from paired tissue samples of n=12 patients and were differentiated into adipocytes in vitro. Expression profile of progenitor cells was assessed by gene array analysis. Real-time PCR was performed to assess mRNA expression of selected genes.

Results Progenitor cells isolated from deep neck and subcutaneous adipose tissue show marked differences in gene expression pattern. In 355 differentially regulated (<1.5 fold) genes, we found genes encoding 32 transcriptions factors, 25 surface proteins and 14 receptor ligands. Validation by qPCR confirmed ADH1B as highest expressed in deep neck progenitor cells. In vitro differentiated adipocytes from the deep neck adipose tissue were characterized by elevated UCP1 and classical brown marker gene expression.

Conclusion The ability of human adipocyte progenitor cells to differentiate into brown-like adipocytes is depot-specific and is based on intrinsic differences in gene expression. Our data provide potential molecular targets involved in the genetic determination of brown adipocyte precursor cells.

Nothing to Disclose: DT, VS, TW, MS, TB, PM, TF, MK, PF, MW

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Sources of Research Support: Ministry of Science, Research and the Arts of Baden-Württemberg (Az: 32-7533.-6-10/15/5); German Federal Ministry for Education and Research (BMBF, project funding reference number (FKZ: 01GI1326) and is integrated in the Competence Network Obesity (CNO).