Session: SUN 0875-0890-Adipocyte Biology-Clinical studies
Poster Board SUN-0888
Methods Paired deep neck and subcutaneous adipose tissue samples from n=52 subjects were taken and subdivided for analysis by immunohistochemistry (n=26) and quantitative real-time PCR (n=14), respectively. Progenitor cells were isolated from paired tissue samples of n=12 patients and were differentiated into adipocytes in vitro. Expression profile of progenitor cells was assessed by gene array analysis. Real-time PCR was performed to assess mRNA expression of selected genes.
Results Progenitor cells isolated from deep neck and subcutaneous adipose tissue show marked differences in gene expression pattern. In 355 differentially regulated (<1.5 fold) genes, we found genes encoding 32 transcriptions factors, 25 surface proteins and 14 receptor ligands. Validation by qPCR confirmed ADH1B as highest expressed in deep neck progenitor cells. In vitro differentiated adipocytes from the deep neck adipose tissue were characterized by elevated UCP1 and classical brown marker gene expression.
Conclusion The ability of human adipocyte progenitor cells to differentiate into brown-like adipocytes is depot-specific and is based on intrinsic differences in gene expression. Our data provide potential molecular targets involved in the genetic determination of brown adipocyte precursor cells.
Nothing to Disclose: DT, VS, TW, MS, TB, PM, TF, MK, PF, MW
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