Session: MON 0405-0434-Novel Pathways in Steroid Hormones Action
Poster Board MON-0432
Fifty μM of Progesterone treatment resulted in 42% cell growth compared to 100% growth in untreated control. But, 3-MA addition partially rescued cell growth to 46.7%. When both treated and rescued cells were allowed to recover initially for 48 hrs, P-50 μM treated cells showed improvement in cell growth to 50%, whereas 3-MA rescued cells showed 57.5% growth. Again, when both cells were allowed to recover for second 48 hrs, rescued cells showed a cell growth of 87%, whereas treated cells maintained at 52.9%, the previous level of growth. So rescued cells recovered cell growth better than treated cells. Partial recovery of cell growth in rescued cells prompted us to check adhesion function. P-50 μM treatment resulted in 71.8% adhesion compared to 100% in untreated control. But, 3-MA partially rescued adhesion to 76.9%. When both treated and rescued cells were allowed to recover for first 48 hrs, rescued cells showed improved adhesion (86.5%) than treated cells (71%). Again, when both cells were allowed to recover for second 48 hrs, rescued and treated cells showed almost equal recovery in adhesion 91.2% and 87.9% respectively. Following adhesion, migration function was checked. P-50 μM treatment decreased migration to 23% compared to 100% in untreated control. But 3-MA partially rescued migration to 45%. However, initial 48 hrs recovery did not improve migration function in both treated and rescued cells.
In conclusion, progesterone treatment decreased in-vitro cell growth, adhesion and migration functions of BLM cells. But 3-MA partially rescued cell growth, adhesion and migration functions. 3-MA rescued cells showed better recovery of in-vitro functions than progesterone treated cells.
Nothing to Disclose: DCL, KJ, PR
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