LB-PP02-1 Comparison of Gut Microbiota in Obese, Diabetic and Healthy Control Individuals

Program: Late-Breaking Abstracts
Session: LBSU 1074-1087-Diabetes & Obesity
Sunday, June 22, 2014: 1:00 PM-3:00 PM
Hall F (McCormick Place West Building)

Poster Board LBSU-1076
Yalcin Basaran1, Abdullah Taslipinar1, Sinasi Erol Bolu1, Mehmet Ali Saracli1, Turker Turker1, Coskun Meric1, Cem Haymana1, Kamil Baskoy1, Mustafa Dinc1, Ferhat Deniz2, Mahmut Yazici1, Aydogan Aydogdu1, Alper Sonmez1 and Omer Azal1
1Gulhane Military Medical Academy School of Medicine, Ankara, Turkey, 2GATA HAYDARPASHA Training Hospital, Istanbul, Turkey
The worldwide prevalence of obesity and type 2 diabetes is a growing epidemic problem. The accumulating evidence indicates that, in addition to genetic susceptibility and environmental factors, changes in the gut microbial composition may be responsible for that increase. The human microbiota consist of as many as 10 to 100 trillion microorganisms. This represents at least 10 fold more cells than those in the human body. It is predicted that each individual harbors at least 160 such species from a total of 1000 to 1150 prevalent bacterial species.

We designed a prospective study to identify the relation between the gut microbiota composition and these metabolic conditions. 27 morbidly obese individuals, 26 patients with newly diagnosed diabetes and 28 healthy control subjects were included in the present study. Fecal samples of the participants were analyzed by quantitative real-time PCR for the presence of Bacteroidetes, Firmicutes, Bifidobacteria (Actinobacteria) and Clostridium Leptum (Firmicutes).

Bacteroidetes concentrations were similar between the three groups and there were no significant differences in the fecal Bifidobacteria, Firmicutes and Clostridium Leptum levels among the obesity and diabetic groups. However, Bifidobacteria, Firmicutes and Clostridium Leptum counts were significantly lower in patients with obesity and diabetes, compared to healthy control individuals. Logistic regression analysis showed that metabolic parameters, such as BMI and HbA1c, waist circumference and HbA1c, and finally weight and FBG were independent risk factors for reduced proportions of Firmicutes, Bifidobacteria and Clostridium Leptum, respectively.

These findings support that both obesity and diabetes may be associated with compositional changes in the intestinal microbial composition. All these results suggest that the gut microbiota can be used as an important marker, helping to determine the risk and etiopathogenesis of aforementioned metabolic disorders.

Nothing to Disclose: YB, AT, SEB, MAS, TT, CM, CH, KB, MD, FD, MY, AA, AS, OA

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