Neonatal Nutritional Programming Impairs Hypothalamic Responsiveness to Adiponectin

Program: Abstracts - Orals, Poster Previews, and Posters
Session: FRI 562-582-Obesity: Basic Science
Friday, March 6, 2015: 1:00 PM-3:00 PM
Hall D-F, Obesity (San Diego Convention Center)

Poster Board FRI-576
Mariana Peduti Halah1, Paula Beatriz Marangon, MSc2, Jose Antunes-Rodrigues, MD, PhD3 and Lucila Elias, MD, PhD3
1University of Sao Paulo, Ribeirao Preto Medical School, Ribeirao Preto, Brazil, 2School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Brazil, 3Ribeirao Preto Medical School - University of Sao Paulo, Ribeirao Preto, Brazil
Background: Exposure to changes in nutritional conditions during prenatal or neonatal life induces long-lasting metabolic effects in adult life.  Adiponectin influences food intake and body weight.  Thus, the aim of this work was to investigate the effects of neonatal nutritional programming on the responsiveness to adiponectin in adult life. Methods: Male Wistar rats were subdivided, just right after birth, in litters of different size: 3 rats (Small litters, SL), 10 rats (Normal litters, NL) or 16 rats (Large litters, LL). After weaning animals received regular diet ad libitum. Pre- and post- weaning body weight gain was evaluated for 60 days. Another set of rats, 53 days after birth, was subjected to cannula implantation into the lateral ventricle. 7 days after surgery, diet was removed at 4pm and rats received icv injection of adiponectin (1μg/rat) or saline at 5pm. Diet was presented 60 min after the injections and food intake and body weight were determined 12h and 24h after. Results: SL rats showed higher  body gained (g) at weaning (day 21: NL: 52.9g ± 1.25; SL: 72.5 ± 2.96; LL: 37.9 ± 2.8) and also thereafter (day 60: NL: 409.4 ± 13.7, SL: 442.2 ± 19.4 and LL: 369.1 ± 11.6, while LL rats remained smaller than control rats during the evaluated periods (p<0.05). Central adiponectin stimulation decreased body weight gain (g) (Vehicle 12h: 18 ± 1.8 and 24h: 7.1 ± 2.1; Adiponectin 12h -3.7 ± 3.8 and 24h: -11.5  ± 6.1) and food intake (g/100g bw) (Vehicle 12h: 9.5± 0.7  and 24h: 12.2± 1.7; Adiponectin 12h: 5.07 ± 0.87 and 24h: 7.7 ± 1.7) in NL rats. However, adiponectin had no effect on SL rats (Food intake: Vehicle 12h: 7.44 ± 1.51 and 24h: 12.8 ± 1.32; Adiponectin 12h: 8.46  ± 0.6 and 24h: 7.68 ± 0.51; Weight gain: Vehicle 12h: 14.75 ± 8.6 and 24h: 4 ± 2; Adiponectin 12h: 13.5 ± 4.28 and 24h: 7.83 ± 1.22) and LL (Food intake: Vehicle 12h: 9.16 ± 1.32 and 24h: 12.32 ± 1.15; Adiponectin 12h: 8.18  ± 1.13 and 24h: 6.8 ± 1.57 ; Weight gain: Vehicle 12h: 17.2 ± 3.34 and 24h: 6.8 ± 2; Adiponectin 12h: 23.8 ± 6.3 and 24h: 13 ± 5.6), compared to the respective vehicle. Conclusion: In conclusion, neonatal over- and undernutrition induces changes in feeding behavior and body weight and impair the responsiveness to central stimulation with adiponectin in adulthood.

Nothing to Disclose: MPH, PBM, JA, LE

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Sources of Research Support: Fapesp and CNPq