Session: SUN 203-235-Steroid Hormone Actions, Biosynthesis and Metabolism (posters)
Bench to Bedside
Poster Board SUN 212
Summary of Mass Spectra data
Compound M+1 M+23 M-183 184 M+1 M+23 M-141 184
DHEA-PC 454 476 271 Yes - - - -
C-341 524 544 341 Yes E-341 482 504 341 No
C-339 522 542 339 Yes E-339 480 502 339 No
C-337 520 540 337 Yes E-337 478 500 337 No
The HPLC system that we used separates the C-compounds from the E-compounds but does not completely resolve them. Although we have not yet completely purified each of these compounds, the LC-MS patterns of each set of fragments have the same retention time. C-339 and C-337 are consistent with unreduced intermediates from the 7-dehydrosterol precursor. These six compounds fit together to form a pathway to C-341, the endogenous digoxin-like material.
There are two known pathways for the biosynthesis of phosphocholine esters: [a] a salvage pathway from other phosphocholine esters and [b] a de novo pathway starting from CDP-serine with a phospho-ethanolamine ester intermediate. Based on the occurrence of the phosphoethanolamine esters, the de novo pathway must be in use. This is important because it indicates that the biochemical process is taking place in the adrenals.
In summary, we have isolated six novel compounds that together form the biosynthetic pathway to C-341, the endogenous digoxin-like material. This is also the first report of the isolation of steroid phosphoethanolamine esters.
Nothing to Disclose: FIC
*Please take note of The Endocrine Society's News Embargo Policy at https://www.endocrine.org/news-room/endo-annual-meeting/pr-resources-for-endo
See more of: Abstracts - Orals, Poster Previews, and Posters