Session: SUN 203-235-Steroid Hormone Actions, Biosynthesis and Metabolism (posters)
Bench to Bedside
Poster Board SUN 220
SHBG was internalised by LNCaP cells and uptake was not androgen dependent. In testosterone uptake assays, intracellular levels of testosterone peaked at 1 hour and thereafter increasing amounts of glucuronidated-testosterone was effluxed from the cell until almost 95% of testosterone was glucuronidated at 24 hours. In the presence of SHBG, only 7% of testosterone was glucuronidated at 24 hours suggesting that SHBG may have a protective effect. As expected, testosterone induced the expression of prostate specific antigen (PSA) mRNA. However addition of SHBG, rather than limiting testosterone function, further increased the testosterone-induced expression of PSA mRNA and further enhanced the testosterone-induced decrease in androgen receptor mRNA. The results strongly suggest that, as well as being the major serum carrier of sex hormones, SHBG is endocytosed and plays a role in the intracellular regulation of androgen action.
Nothing to Disclose: HL, TP, BM, JPU, CJP, DJR, RHM, MCD, KR
*Please take note of The Endocrine Society's News Embargo Policy at https://www.endocrine.org/news-room/endo-annual-meeting/pr-resources-for-endo
See more of: Abstracts - Orals, Poster Previews, and Posters